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Real-time monitoring of biomolecular interactions in blood plasma using a surface plasmon resonance biosensor

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Abstract

We report a novel approach to biosensor-based observations of biomolecular interactions which enables real-time monitoring of biomolecular interactions in complex media. This approach is demonstrated by investigating the interaction between the human chorionic gonadotropin (hCG) and its antibody in blood plasma using a surface plasmon resonance biosensor and a dispersionless microfluidics system. The real-time binding data obtained in blood plasma are compared with those obtained in buffer and blood plasma using a conventional method. It is also demonstrated that the proposed approach can enhance the capability of the biosensor to detect biomolecules in complex samples in terms of detection time and sensitivity. In the model experiment, this approach is shown to enable direct detection of hCG in blood plasma at levels which are five times lower than those detected using the conventional detection approach.

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Acknowledgment

This work was supported by the Academy of Sciences of the Czech Republic (grant KAN200670701) and by the Ministry of Education, Youth and Sports of the Czech Republic (grant OC09058). Authors would like to acknowledge the Institute of Hematology and Blood Transfusion, Prague, for providing blood plasma samples used in this research.

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Authors and Affiliations

  1. Department of Optical Sensors, Institute of Photonics and Electronics AS CR, v. v. i., Chaberská 57, 182 51, Prague, Czech Republic

    Tomáš Špringer, Marek Piliarik & Jiří Homola

Authors
  1. Tomáš Špringer
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  2. Marek Piliarik
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  3. Jiří Homola
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Corresponding author

Correspondence to Jiří Homola.

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Špringer, T., Piliarik, M. & Homola, J. Real-time monitoring of biomolecular interactions in blood plasma using a surface plasmon resonance biosensor. Anal Bioanal Chem 398, 1955–1961 (2010). https://doi.org/10.1007/s00216-010-4159-9

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  • DOI: https://doi.org/10.1007/s00216-010-4159-9

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