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Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food

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Abstract

We describe the development of a six-target real-time multiplex PCR assay with the SYBR® GreenER™ fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin.

Multiplex real-time PCR for the detection of DNA allergens in food using ABI Prism® 7000 Sequence detection system

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Acknowledgements

This study was carried out with the financial support of Lombardia (IT) regional project METADISTRETTI, of the University of Parma (FIL), of Emilia-Romagna (IT) regional project SITEIA, and of TRACE-BACK (FP6-2005-FOOD-036300). TRACE-BACK is a project supported by the European Commission Sixth Framework Programme. The content of this paper does not necessarily reflect the Commission of the European Communities’ views and in no way anticipates the Commission’s future policy in this area. We thank Dr Robert Knoeber for reviewing the language and for fruitful comments.

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Authors and Affiliations

  1. Department of Environmental Sciences, Division of Genetics and Environmental Biotechnology, University of Parma, Viale G.P. Usberti 11/A, 43100, Parma, Italy

    Simona Pafundo, Mariolina Gullì & Nelson Marmiroli

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  1. Simona Pafundo
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  2. Mariolina Gullì
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  3. Nelson Marmiroli
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Correspondence to Nelson Marmiroli.

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Pafundo, S., Gullì, M. & Marmiroli, N. Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food. Anal Bioanal Chem 396, 1831–1839 (2010). https://doi.org/10.1007/s00216-009-3419-z

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  • DOI: https://doi.org/10.1007/s00216-009-3419-z

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