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Detection of angiotensin II type 1 receptor ligands by a cell-based assay

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Abstract

This work describes a cell-based assay that does not depend on radioactivity or laboratory animals for the detection of ligands of angiotensin II type 1 receptor (AT1R). The assay makes use of stable transfected Chinese hamster ovary cells (CHO-AT1R) expressing the AT1R. A sequential saturation assay principle was used in which receptor binding sites of the CHO-AT1R cells are blocked by the analyte in a concentration-dependent manner. Afterwards, TAMRA-angiotensin II, a fluorescence-labeled ligand, was added to bind to the remaining free binding sites of the receptor. In consequence, the fluorescence signal determined is inversely proportional to the concentration of the analyte.

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Abbreviations

AngII:

angiotensin II

AT1R:

angiotensin II type 1 receptor

CHO-AT1R:

Chinese hamster ovary cells stably expressing exogenous angiotensin II type 1 receptor

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Acknowledgement

The project was supported by BMBF grant FKZ 03IP515.

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Authors and Affiliations

  1. Fraunhofer Institute for Biomedical Engineering (IBMT), Am Mühlenberg 13, 14476, Potsdam, Germany

    Matthias Grießner, Eva Ehrentreich-Förster & Frank F. Bier

  2. Institute of Biochemistry and Biology, iPOC Research Group, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Golm, Germany

    Patrick Bröker & André Lehmann

Authors
  1. Matthias Grießner
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  2. Patrick Bröker
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  3. André Lehmann
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  4. Eva Ehrentreich-Förster
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  5. Frank F. Bier
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Corresponding author

Correspondence to Matthias Grießner.

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Grießner, M., Bröker, P., Lehmann, A. et al. Detection of angiotensin II type 1 receptor ligands by a cell-based assay. Anal Bioanal Chem 395, 1937–1940 (2009). https://doi.org/10.1007/s00216-009-3074-4

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  • DOI: https://doi.org/10.1007/s00216-009-3074-4

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