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Immobilized monolithic enzyme reactors for application in proteomics and pharmaceutics

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Abstract

The use of monolithic supports for a wide variety of applications has rapidly expanded during the past few years. The examples for applications of monoliths presented herein show that the chromatographic performance of bioreactors and affinity media prepared from monolithic media is superior to that of conventional particle-based systems. The ease of fabrication and modification combined with the long lifetime of the monolithic columns and their potential to be used in fully automated analytical systems make them attractive tools for an increasing number of applications.

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Abbreviations

ASP:

affinity stationary phase

CYP:

cytochrome P450

ESI-MS/MS:

electrospray ionization tandem mass spectrometry

HPLC:

high-performance liquid chromatography

IMER:

immobilized monolithic enzyme reactor

MS:

mass spectrometry

MSP:

monolithic stationary phase

PEGMA:

polyethoxylated hydroxyethyl methacrylate

RP:

reversed phase

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Acknowledgement

The authors thank A. Wolfsteller for providing scanning electron microscopy images. M. Müller is acknowledged for graphical assistance.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Wolfgang-Langenbeck-Straße 4, 06120, Halle, Saale, Germany

    Jens Sproß & Andrea Sinz

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  1. Jens Sproß
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  2. Andrea Sinz
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Correspondence to Andrea Sinz.

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Sproß, J., Sinz, A. Immobilized monolithic enzyme reactors for application in proteomics and pharmaceutics. Anal Bioanal Chem 395, 1583–1588 (2009). https://doi.org/10.1007/s00216-009-2998-z

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  • DOI: https://doi.org/10.1007/s00216-009-2998-z

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