Abstract
Surface wettability conversion with hydrophobins is important for its applications in biodevices. In this work, the application of a type I hydrophobin HGFI in surface wettability conversion on mica, glass, and poly(dimethylsiloxane) (PDMS) was investigated. X-ray photoelectron spectroscopy (XPS) and water-contact-angle (WCA) measurements indicated that HGFI modification could efficiently change the surface wettability. Data also showed that self-assembled HGFI had better stability than type II hydrophobin HFBI. Protein patterning and the following immunoassay illustrated that surface modification with HGFI should be a feasible strategy for biosensor device fabrication.
A hydrophobin HGFI has been applied into surface wettability conversion for protein immobilization
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References
Yam CM, Mayeux A, Milenkovic A, Cai CZ (2002) Langmuir 18:10274–10278
Hektor HJ, Scholtmeijer K (2005) Curr Opin Biotech 16:434–439
Wessels JGH, de Vries OMH, Asgeirsdottir SA, Schuren FHJ (1991) Plant Cell 3:793–799
Wosten HAB (2001) Annu Rev Microbiol 55:625–646
Wessels JGH (1994) Annu Rev Phytopathol 32:413–437
Scholtmeijer K, Janssen MI, Gerssen B, de Vocht ML, Leeuwen BM, von Kooten TG, Wosten HA, Wessels JG (2002) Appl Environ Microbiol 68:1367–1373
de Vocht ML, Reviakine I, Wosten HA, Brisson A, Wessels JG, Robillard GT (2000) J Biol Chem 275:28428–28432
Torkkeli M, Serimaa R, Ikkala O, Linder M (2002) Biophys J 83:2240–2247
Ritva S, Torkkeli M, Paananen A, Linder M, Kisko K, Knaapila M, Ikkala O, Vuorimaa E, Lemmetyinend H, Seecke O (2003) J Appl Crystallogr 36:499–502
de Vries OMH, Fekkes MP, Wosten HAB, Wessels JGH (1993) Arch Microbiol 159:330–335
Carpenter CE, Mueller RJ, Kazmierczak P, Zhang L, Villalon DK, van Alfen NK (1992) Mol Plant Microbe Interact 5:55–61
Sarno DM, Murphy AV, DiVirgilio ES, Jones WE, Ben RN (2003) Langmuir 19:4740–4744
Wang LK, Feng XZ, Hou S, Chan QL, Qin M (2006) Surface Interface Anal 38:44–50
Qin M, Hou S, Wang LK, Feng XZ, Wang R, Yang YL, Wang C, Liu L, Shao B, Qiao MQ (2007) Colloids and Surfaces B Biointerfaces 60:243–249
Park JH, Park KD, Bae YH (1999) Biomaterials 20:943–953
Marois Y, Sigot-Luizard MF, Guidoin R (1999) ASAIO J 45:272–280
Ertel SI, Ratner BD, Kaul A, Schway MB, Horbett TA (1994) J Biomed Mater Res 28:667–675
van-Kooten TG, Whitesides JF, von Recum AF (1998) J Biomed Mater Res 43:1–14
Interrante LV, Shen Q, Li J (2001) Macromolecules 34:1545–1547
Dahrouch M, Schemidt A, Leemans L, Linssen H, Goetz H (2003) Macromol Symp 199:147–162
Wessels JG (1997) Adv Microb Physiol 38:1–45
Genzer J, Efimenko K (2000) Science 290:2130–2133
Wilson DJ, Pond RC, Williams RL (2000) Interface Sci 8:389–399
Xia Y, Whitesides GM (1998) Angew Chem Int Ed Engl 37:550–575
Khorasani MT, Mirzadeh H, Sammes PG (1999) Radiat Phy Chem 55:685–689
Okaniwa M, Ohta Y (1997) J Polym Sci Part A Polym Chem 35:2607–2617
Yu L, Zhang B, Szilvay GR, Sun R, Janis J, Wang Z, Feng S, Xu H, Linder MB, Qiao M (2008) Microbiology 154:1677–1685
Kumar A, Whitesides GM (1993) Appl Phys Lett 63:2002–2004
Wenzel RN (1936) Ind Eng Chem 28:988–990
Wang R, Yang YL, Qin M, Wang LK, Yu L, Shao B, Qiao MQ, Wang C, Feng XZ (2007) Chem Mater 19:3227–3231
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This work has been supported by the National Natural Science Foundation of China (Grant number: 90403140).
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Hou, S., Li, X., Li, X. et al. Surface modification using a novel type I hydrophobin HGFI. Anal Bioanal Chem 394, 783–789 (2009). https://doi.org/10.1007/s00216-009-2776-y
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DOI: https://doi.org/10.1007/s00216-009-2776-y