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Rapid detection of Mycobacterium tuberculosis cells by using microtip-based immunoassay

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Abstract

This paper describes a microtip-based approach of concentrating target analytes for a highly sensitive bioassay. As an example, rapid screening of bacterial whole cells is presented to detect Mycobacterium tuberculosis (MTB), a pathogenic bacterium for human tuberculosis (TB). The concentration and detection is performed with three sequential steps of (1) attracting bacterial whole cells in the vicinity of a microtip by alternating current electroosmotic flow; (2) capturing the cells onto the microtip by capillary action; (3) binding fluorophore-labeled polyclonal antibodies to the cells followed by fluorescence measurement (immunofluorescence). Through this mechanism, MTB cells have been detected to the concentration of 8,000 cells/mL within 10 min. This sensitivity is comparable to that of Ziehl–Neelsen smear microscopy, a common culture-free screening method for diagnosis of TB. For comparison, Escherichia coli O157:H7 cells have also been detected to the concentration of 30,000 cells/mL in the same way.

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References

  1. WHO (2006) Diagnostics for tuberculosis-global demand and market potential. Geneva, Switzerland

    Google Scholar 

  2. Chan ED, Reves R, Belisle JT, Brennan PJ, Hahn WE (2000) Am J Respir Crit Care Med 161:1713–1719

    CAS  Google Scholar 

  3. Cheng VCC, Yew WW, Yuen KY (2005) Eur J Clin Microbiol Infect Dis 24:711–720

    Article  CAS  Google Scholar 

  4. Van Deun A, Portaels F (1998) Int J Tuberc Lung Dis 2:756–765

    Google Scholar 

  5. Sada E, Aguilar D, Torres M, Herrera TJ (1992) J Clin Microbiol 30:2415–2418

    CAS  Google Scholar 

  6. Arias-Bouda LMP, Nguyen LN, Ho LM, Kuijper S, Jansen HM, Kolk AHJ (2000) J Clin Microbiol 38:2278–2283

    CAS  Google Scholar 

  7. GlatmanFreedman A, Martin JM, Riska PF, Bloom BR, Casadevall A (1996) J Clin Microbiol 34:2795–2802

    CAS  Google Scholar 

  8. Araj GF, Fahmawi BH, Chugh TD, Abusalim M (1993) Diagn Microbiol Infect Dis 17:119–127

    Article  CAS  Google Scholar 

  9. Attallah AM, Malak CAA, Ismail H, El-Saggan AH, Omran MM, Tabll AA (2003) J Immunoassay Immunochem 24:73–87

    Article  CAS  Google Scholar 

  10. Diaz-Gonzalez M, Gonzalez-Garcia MB, Costa-Garcia A (2005) Biosens Bioelectron 20:2035–2043

    Article  CAS  Google Scholar 

  11. Lyashchenko KP, Singh M, Colangeli R, Gennaro ML (2000) J Immunol Methods 242:91–100

    Article  CAS  Google Scholar 

  12. Pottumarthy S, Wells VC, Morris AJ (2000) J Clin Microbiol 38:2227–2231

    CAS  Google Scholar 

  13. Sheehan PE, Whitman LJ (2005) Nano Lett 5:803–807

    Article  CAS  Google Scholar 

  14. Wong PK, Chen CY, Wang TH, Ho CM (2004) Anal Chem 76:6908–6914

    Article  CAS  Google Scholar 

  15. Trau M, Saville DA, Aksay IA (1997) Langmuir 13:6375–6381

    Article  CAS  Google Scholar 

  16. Pohl HA, Hawk I (1966) Science 152:647–649

    Article  Google Scholar 

  17. Liu Y, Liu WK, Belytschko T, Patankar N, To AC, Kopacz A, Chung JH (2007) Int J Numer Methods Eng 71:379–405

    Article  Google Scholar 

  18. Pan B, Cui D, Xu P, Li Q, Huang T, He R, Gao F (2007) Colloid Surf A 295:217–222

    Article  CAS  Google Scholar 

  19. Gray DS, Tan JL, Voldman J, Chen CS (2004) Biosens Bioelectron 19:1765–1774

    Article  CAS  Google Scholar 

  20. Saha K (2003) Langmuir 19:1304–1311

    Article  CAS  Google Scholar 

  21. Shen AQ, Gleason B, McKinley GH, Stone HA (2002) Phys Fluids 14:4055–4068

    Article  CAS  Google Scholar 

Download references

Acknowledgment

The authors acknowledge the support of a CDC contract (200-2007-M-22794, Program Manager: David A. Wilson at Division of TB Elimination/Nat. Center for HIV, Viral Hepatitis, STD & TB/Centers for Disease Control and Prevention).

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Authors and Affiliations

  1. Department of Mechanical Engineering, University of Washington, Campus Box 352600, Seattle, WA, 98195, USA

    Woon-Hong Yeo, Shieng Liu & Jae-Hyun Chung

  2. Department of Mechanical and Aerospace Engineering, University of Texas at Arlington, Box 19018, Arlington, TX, 76019, USA

    Yaling Liu

  3. NanoFacture, Inc., 3983 Research Park Dr Suite 100, Ann Arbor, MI, 48108, USA

    Kyong-Hoon Lee

Authors
  1. Woon-Hong Yeo
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  2. Shieng Liu
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  3. Jae-Hyun Chung
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  4. Yaling Liu
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  5. Kyong-Hoon Lee
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Corresponding author

Correspondence to Kyong-Hoon Lee.

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Yeo, WH., Liu, S., Chung, JH. et al. Rapid detection of Mycobacterium tuberculosis cells by using microtip-based immunoassay. Anal Bioanal Chem 393, 1593–1600 (2009). https://doi.org/10.1007/s00216-008-2591-x

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  • DOI: https://doi.org/10.1007/s00216-008-2591-x

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