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Ultra performance liquid chromatography tandem mass spectrometry performance evaluation for analysis of antibiotics in natural waters

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Abstract

An ultra performance liquid chromatography electrospray tandem mass spectrometry (UPLC/MS/MS) method was developed and validated for the determination of 17 antibiotics in natural waters in one single extraction and chromatographic procedure. Gradient separation conditions were optimised for 17 compounds belonging to five different antibiotic groups: quinolones (oxolinic acid, nalidixic acid, pipemidic acid, flumequine), fluoroquinolones (enoxacin, ciprofloxacin, norfloxacin, ofloxacin, enrofloxacin, sarafloxacin, danofloxacin, difloxacin, lomefloxacin), sulphonamides (sulphamethoxazole, sulphamethazine), nitro-imidazole (ornidazole) and diaminopyrimidine (trimethoprim). The separation of all compounds, obtained using a 1.7 μm particle size column (100 mm × 2.1 mm), was achieved within 10 min time. Water samples were adjusted to pH 7 and extracted using Oasis hydrophilic–lipophilic balance (HLB) solid phase extraction cartridges. After elution with methanol and concentration, extracts were injected in a C18 column (Acquity UPLC BEH C18) and detected by tandem mass spectrometry. Average recovery from 100 ng L−1 fortified samples was higher than 70% for most of the compounds, with relative standard deviations below 20%. Performances of the method (recoveries, detection limit, quantification limit and relative standard deviation) and matrix effects were studied, and results obtained showed that method was suitable for routine analysis of antibiotics in surface water. Samples analysis from Seine River (France) confirmed the interest of antibiotic contamination evaluation in that area.

UPLC/MS/MS extracted ion chromatograms of a standard solution containing 17 analytes

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Acknowledgements

This research was financed by a doctoral grant from the Ile-de-France region. Public and private institutions associated with PIREN-Seine are gratefully acknowledged for their financial support. The authors thank Annie Desportes, Claire Arcelin for help in laboratory work and Olivier Thomas for helpful comment on the manuscript and English correction.

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Authors and Affiliations

  1. Laboratoire Hydrologie et Environnement, EPHE, UMR Sisyphe 7619, Université Pierre et Marie Curie, 4 place Jussieu, BC 105, 75252, Paris Cedex, France

    Fatima Tamtam, Joëlle Eurin & Marc Chevreuil

  2. Ecole des Hautes Etudes en Santé Publique (EHESP), Laboratoire d’Etude et de Recherche en Environnement et Santé (LERES), Avenue du Professeur Léon-Bernard, 35043, Rennes cedex, France

    Fabien Mercier & Barbara Le Bot

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  1. Fatima Tamtam
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  2. Fabien Mercier
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Correspondence to Fatima Tamtam.

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Tamtam, F., Mercier, F., Eurin, J. et al. Ultra performance liquid chromatography tandem mass spectrometry performance evaluation for analysis of antibiotics in natural waters. Anal Bioanal Chem 393, 1709–1718 (2009). https://doi.org/10.1007/s00216-008-2576-9

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  • DOI: https://doi.org/10.1007/s00216-008-2576-9

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