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Towards biosensors based on conducting polymer nanowires

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Abstract

We report the electrochemical deposition of poly(pyrrolepropylic acid) nanowires, their covalent modification with antibodies and their conversion into potential functional sensor devices. The nanowires and the devices were characterised by optical microscopy, fluorescence microscopy, electron microscopy and electrical measurements. Fluorescence images, current–voltage (I–V) profiles and real-time sensing measurements demonstrated a rapid and highly sensitive and selective detection of human serum albumin (HSA), a substance that has been used to diagnose incipient renal disease. The detection is based on the selective binding of HSA onto anti-HSA that is covalently attached to the nanowires. The binding changes the electrical properties of the nanowires thus enabling the real-time detection. Whilst the utility of the research was demonstrated for protein binding/detection, the technology could easily be designed for the detection of other analytes by the modification of polymer nanowires with other analyte-specific molecules/biomolecules. Therefore, the technology has the potential to positively impact broad analytical applications in the biomedical, environmental and other sectors.

Real-time dynamic current response on sequential exposure of buffer, bovine serum albumin (BSA) and human serum albumin (HSA) onto anti-HSA modified poly (pyrrolepropylic acid) nanowires. Fluorescence images of poly(pyrrolepropylic acid) nanowire (top right) and polypyrrole nanowire control (bottom right) after sequential treatment with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), anti HSA and fluorophore-labeled HSA.

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Acknowledgements

We greatly acknowledge support by the Missouri State University Faculty Summer Fellowship.

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Authors and Affiliations

  1. Department of Chemistry, Missouri State University, Springfield, MO, 65897, USA

    Sagar B. Tolani & Adam K. Wanekaya

  2. Department of Biomedical Science, Missouri State University, Springfield, MO, 65897, USA

    Michael Craig & Robert K. DeLong

  3. Department of Physics, Missouri State University, Springfield, MO, 65897, USA

    Kartik Ghosh

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  1. Sagar B. Tolani
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  2. Michael Craig
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  3. Robert K. DeLong
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  4. Kartik Ghosh
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  5. Adam K. Wanekaya
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Correspondence to Adam K. Wanekaya.

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Tolani, S.B., Craig, M., DeLong, R.K. et al. Towards biosensors based on conducting polymer nanowires. Anal Bioanal Chem 393, 1225–1231 (2009). https://doi.org/10.1007/s00216-008-2556-0

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  • DOI: https://doi.org/10.1007/s00216-008-2556-0

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