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Rapid determination of five antibiotic residues in swine wastewater by online solid-phase extraction–high performance liquid chromatography–tandem mass spectrometry

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Abstract

A simple and quick online solid-phase extraction (SPE) coupled to liquid chromatography (LC)/tandem mass spectrometry (MS/MS) for the determination of the five antibiotics (florfenicol, FF; lincomycin, LCM; oxytetracyclin, OTC; tylosin, TS; valnemulin, VLM) in swine wastewater has been developed. After filtration, aliquots (100 μl) of wastewater samples were directly injected to a column-switching LC system. Some matrix interference was removed by washing up SPE column with 0.2% formic acid solution and acetonitrile. Antibiotics eluted from SPE column were separated on analytical column by converting switching valve and were detected by MS/MS. Calibration curves using the method of standard addition had very good correlation coefficients (r > 0.99) in the range of 0.1 to 2 ng/ml. The intra-day precision of the method was less than 12% and the inter-day precision was between 6 to 17%. The detection limits were 0.01–0.1 ng/ml. When this method was applied to wastewater samples in swine facilities, four compounds (LCM, OTC, TS, and VLM) were detected.

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Authors and Affiliations

  1. Institute of Public Health and Environment, Miyagi Prefectural Government, 4-7-2 Saiwai-cho, Miyagino-ku, Sendai, 983-8666, Japan

    Misako Tagiri-Endo, Tomoyuki Nakamura, Takashi Hatakeyama & Kazuo Kawamukai

  2. Tohoku Ryokka Kankyohozen, 3-8-22 Sakuragi, Tagajo, Miyagi, 985-0842, Japan

    Shigeru Suzuki

Authors
  1. Misako Tagiri-Endo
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  2. Shigeru Suzuki
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  3. Tomoyuki Nakamura
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  4. Takashi Hatakeyama
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  5. Kazuo Kawamukai
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Correspondence to Misako Tagiri-Endo.

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Tagiri-Endo, M., Suzuki, S., Nakamura, T. et al. Rapid determination of five antibiotic residues in swine wastewater by online solid-phase extraction–high performance liquid chromatography–tandem mass spectrometry. Anal Bioanal Chem 393, 1367–1375 (2009). https://doi.org/10.1007/s00216-008-2543-5

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  • DOI: https://doi.org/10.1007/s00216-008-2543-5

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