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Optical sensing in microfluidic lab-on-a-chip by femtosecond-laser-written waveguides

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Abstract

We use direct femtosecond laser writing to integrate optical waveguides into a commercial fused silica capillary electrophoresis chip. High-quality waveguides crossing the microfluidic channels are fabricated and used to optically address, with high spatial selectivity, their content. Fluorescence from the optically excited volume is efficiently collected at a 90° angle by a high numerical aperture fiber, resulting in a highly compact and portable device. To test the platform we performed electrophoresis and detection of a 23-mer oligonucleotide plug. Our approach is quite powerful because it allows the integration of photonic functionalities, by simple post-processing, into commercial LOCs fabricated with standard techniques.

Femtosecond laser written waveguides can selectively excite fluorescence in a microfluidic channel of a commercial lab-on-a-chip. A compact scheme for on-chip detection by laser induced fluorescence is applied to capillary electrophoresis of a 23-mer Cy3-labeled oligonucleotide

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Acknowledgements

This work was funded by the European Commission, 6th FP STREP Project Contract No. IST-2005-034562 [Hybrid Integrated Biophotonic Sensors Created by Ultrafast laser Systems (HIBISCUS)].

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Authors and Affiliations

  1. Istituto di Fotonica e Nanotecnologie - CNR, Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci, 32, 20133, Milan, Italy

    Rebeca Martinez Vazquez, Roberto Osellame, Roberta Ramponi & Giulio Cerullo

  2. Istituto di Chimica del Riconoscimento Molecolare - CNR, Via Mario Bianco, 9, 20131, Milan, Italy

    Marina Cretich & Marcella Chiari

  3. Integrated Optical MicroSystems, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands

    Chaitanya Dongre, Hugo J. W. M. Hoekstra & Markus Pollnau

  4. LioniX BV, 7500, AH, Enschede, The Netherlands

    Hans van den Vlekkert

Authors
  1. Rebeca Martinez Vazquez
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  2. Roberto Osellame
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  3. Marina Cretich
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  4. Marcella Chiari
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  5. Chaitanya Dongre
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  6. Hugo J. W. M. Hoekstra
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  7. Markus Pollnau
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  8. Hans van den Vlekkert
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  9. Roberta Ramponi
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  10. Giulio Cerullo
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Corresponding author

Correspondence to Roberto Osellame.

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Martinez Vazquez, R., Osellame, R., Cretich, M. et al. Optical sensing in microfluidic lab-on-a-chip by femtosecond-laser-written waveguides. Anal Bioanal Chem 393, 1209–1216 (2009). https://doi.org/10.1007/s00216-008-2399-8

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  • DOI: https://doi.org/10.1007/s00216-008-2399-8

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