Skip to main content
SpringerLink
Log in

Static–dynamic sequential superheated liquid extraction of phenols and fatty acids from alperujo

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Superheated liquids of different polarity have been used for sequential extraction of fatty acids and phenols from alperujo. Multivariate methodology has been used to optimise the static–dynamic extraction. Forty-two minutes are required to complete extraction (20 mg/kg of fatty acids and up to 2,200 mg/kg of hydroxytyrosol in the raw material used). The efficacy of the extraction has been demonstrated and compared with that of conventional methods (Folch and stirring-based methods for fatty acids and phenols, respectively), which needed 4.5 and 24 h for the extraction of fatty acids and phenols, respectively. The non-polar and polar extracts were injected into GC–MS and HPLC–MS–MS equipment, respectively, for individual separation–quantification of the target compounds. The simplicity of the experimental setup and the low costs of the raw material make the proposed method advisable when extraction of both fractions is required.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Luque de Castro MD, Japón-Luján R (2006) Trends Anal Chem 25:501–510

    Article  CAS  Google Scholar 

  2. Misir R, Laarveld B, Blair R (1985) J Cromatogr 331:141–146

    Article  CAS  Google Scholar 

  3. Olías JM, Lozano MD, Ríos JJ, Gutiérrez F, Gutiérrez R (1988) Grasas y Aceites 39:77–82

    Google Scholar 

  4. Finotti E, Beye C, Nardo N, Quaglia GB, Milin C, Giacometti J (2001) Nahrung 45:350–35

    Article  CAS  Google Scholar 

  5. Graciano J, Cota J (1984) Grasas y Aceites 35:358–361

    Google Scholar 

  6. Priego-Capote F, Ruiz-Jiménez J, Luque de Castro MD (2004) J Chromatogr A 1045:239–246

    Article  CAS  Google Scholar 

  7. D’Imperio M, Dugo G, Alfa M, Mannina L, Segre AL (2007) Food Chem 102:956–960

    Article  CAS  Google Scholar 

  8. Procida G, Ceccon L (2006) Anal Chim Acta 561:103–108

    Article  CAS  Google Scholar 

  9. León L, Rallo L, Garrido A (2003) Grasas y Aceites 54:41–48

    Google Scholar 

  10. Inon FA, Garrigues JM, Garrigues S, Molina A, De la Guardia M (2003) Anal Chim Acta 489:59–64

    Article  CAS  Google Scholar 

  11. Muik B, Lendl B, Molina-Díaz A, Ayora-Canada MJ (2003) Anal Chim Acta 487:211–215

    Article  CAS  Google Scholar 

  12. Bouaziz M, Grayer RJ, Simmonds MSJ, Damak M, Sayadi S (2005) J Agric Food Chem 53:236–242

    Article  CAS  Google Scholar 

  13. Murkovic M, Lechner S, Pietzka A, Bratacos M, Katzogianos E (2004) J Biochem Biophys Methods 61:155–161

    Article  CAS  Google Scholar 

  14. Morales-Muñoz S, Luque-García JL, Luque de Castro MD (2003) Crit Rev Environ Sci Technol 33:391–395

    Article  Google Scholar 

  15. Fernández-Pérez V, Jiménez-Carmona MM, Luque de Castro MD (1999) J Anal At Spectrom 14:1761–1766

    Article  Google Scholar 

  16. Luque-García JL, Luque de Castro MD (2003) J Chromatogr A 1010:129–133

    Article  Google Scholar 

  17. Alonso-Salces RM, Korta E, Barranco A, Berruela LA, Gallo B, Vicente F (2001) J Chromatogr A 933:37–43

    Article  CAS  Google Scholar 

  18. Luque-Rodríguez JM, Luque de Castro MD, Pérez-Juan P (2005) Talanta 68:126–131

    Article  Google Scholar 

  19. Alonso-Salces RM, Korta E, Barranco A, Berruela LA, Gallo B, Vicente F (2001) J Agric Food Chem 49:3761–3766

    Article  CAS  Google Scholar 

  20. Papagiannopoulos M, Wollseifen HR, Mellenthin A, Galesa R (2004) J Agric Food Chem 52:3784–3789

    Article  CAS  Google Scholar 

  21. Ibañez E, Kubatova A, Señoràns FJ, Cavero S, Reglero G, Hawthorne SB (2003) J Agric Food Chem 51:375–379

    Article  Google Scholar 

  22. Folch J, Lees M, Sloane-Stanley GH (1957) Biol Chem 226:497–502

    CAS  Google Scholar 

  23. Massart DL, Vanderginste BGM, Buydens LMC, De Jong S, Lewi PJ, Smeyers-Verbeke J (1997) Handbook of chemometrics and qualimetrics, Part A. Elsevier, Amsterdam

    Google Scholar 

Download references

Acknowledgements

The Junta de Andalucía, through the PAI Excellence programm (project No. FQM-1515) and Spain’s Ministry of Science and Technology (Project No. CTQ2006–01614) are gratefully acknowledged for financial support. J.A. Pérez-Serradilla is also grateful to Spain’s Ministry of Education for award of his research training fellowship.

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, University of Córdoba, Annex Marie Curie Building, Campus of Rabanales, 14071, Córdoba, Spain

    J. A. Pérez-Serradilla, R. Japón-Luján & M. D. Luque de Castro

Authors
  1. J. A. Pérez-Serradilla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Japón-Luján
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. D. Luque de Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. D. Luque de Castro.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pérez-Serradilla, J.A., Japón-Luján, R. & Luque de Castro, M.D. Static–dynamic sequential superheated liquid extraction of phenols and fatty acids from alperujo. Anal Bioanal Chem 392, 1241–1248 (2008). https://doi.org/10.1007/s00216-008-2376-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-008-2376-2

Keywords

Search

Navigation