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Prostate-specific antigen immunoassays on the BioCD

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Abstract

The BioCD is a spinning-disc interferometric biosensor on which antibodies are immobilized to capture target antigens from biological samples. In this work, BioCDs measured the interferometric response to prostate-specific antigen (PSA). The ideal detection limit for PSA was determined using a BioCD with 12,500 printed target antibody spots with a corresponding number of reference protein spots. Statistical analysis projects the detection limit of PSA as a function of the number of spots included in the average. When approximately 10,000 spot pairs were averaged, the 3σ detection limit was 60 pg/ml in a 2 mg/ml simple protein background. A standard format for BioCD immunoassays uses 96 wells with 32 target spots paired with reference spots. In serum, the detection limit for this format was 1 ng/ml in 3:1 diluted female human serum using a sandwich assay with a nonfluorescent mass tag.

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Acknowledgements

This work was sponsored under grants from Quadraspec Inc. and from the Indiana Economic Development Corporation through the Purdue Research Foundation. We thank Sven Schreiber, Benjamin Edquist, and other staff at Quadraspec Inc. for help in printing the antibody microarray and advice on BioCD surface chemistry.

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Authors and Affiliations

  1. Department of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, IN, 47907-2036, USA

    Xuefeng Wang, Ming Zhao & David D. Nolte

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  1. Xuefeng Wang
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  2. Ming Zhao
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  3. David D. Nolte
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Correspondence to Xuefeng Wang.

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Wang, X., Zhao, M. & Nolte, D.D. Prostate-specific antigen immunoassays on the BioCD. Anal Bioanal Chem 393, 1151–1156 (2009). https://doi.org/10.1007/s00216-008-2370-8

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  • DOI: https://doi.org/10.1007/s00216-008-2370-8

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