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A liquid chromatography method using a monolithic column for the determination of corticoids in animal feed and animal feeding water

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Abstract

An HPLC-DAD method for determining corticoids in calf feed and in animal feeding water samples using a monolithic column has been developed and validated. The method optimization included the study of binary mobile phases of water and acetonitrile. The optimum separation was achieved at 40 °C, with acetonitrile:H2O 29:71 v/v used as mobile phase and a 3 ml/min flow-rate, which resulted in their separation in about 5 min. Two reported sample procedures were applied to feed and for animal feeding water samples prior to HPLC. Method validation was carried out according to the EU criteria established for quantitative screening methods. The results indicate that this method is highly specific, reproducible and accurate. The proposed method was found to be robust and unaffected by small variations in the extraction procedure and in HPLC conditions. The developed method for the determination of corticoids in feed and water samples was also found to be suitable for different kinds of feeds and waters.

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Acknowledgments

This work has been supported by Project 07G/047.1/2003, Consejería de Educación de la Comunidad de Madrid. R. Muñiz-Valencia and S. G. Ceballos-Magaña thanks CONACyT for a grant, and the authors also thank A. Cubedo of Nutral for supplying feed samples.

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Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense, 28040, Madrid, Spain

    R. Muñiz-Valencia, S. G. Ceballos-Magaña, R. Gonzalo-Lumbreras & R. Izquierdo-Hornillos

  2. ETSI, Departamento de Ingeniería Mecánica, Universidad Pontificia Comillas, Alberto Aguilera 25, 28015, Madrid, Spain

    A. Santos-Montes

Authors
  1. R. Muñiz-Valencia
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  2. S. G. Ceballos-Magaña
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  3. R. Gonzalo-Lumbreras
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  4. A. Santos-Montes
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  5. R. Izquierdo-Hornillos
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Correspondence to R. Muñiz-Valencia.

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Muñiz-Valencia, R., Ceballos-Magaña, S.G., Gonzalo-Lumbreras, R. et al. A liquid chromatography method using a monolithic column for the determination of corticoids in animal feed and animal feeding water. Anal Bioanal Chem 391, 2683–2691 (2008). https://doi.org/10.1007/s00216-008-2176-8

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  • DOI: https://doi.org/10.1007/s00216-008-2176-8

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