Abstract
The strontium content of serum, bone, marrow, and teeth was determined by inductively-coupled plasma mass spectrometry (ICP–MS). Significant correlations were obtained after the data were subjected to quality assurance (QA) performed according to validated procedures. After four weeks of treatment with strontium malonate, strontium levels increased from 76 ± 9 μg g−1 in placebo-treated dogs to levels of 7.2 ± 1.7 mg g−1, 9.5 ± 2.7 mg g−1, and 9.8 ± 2.7 mg g−1 in groups treated with 300, 1000, and 3000 mg kg−1 day−1, respectively. Strontium induced a highly significant increase in the bone formation marker, bone-specific alkaline phosphatase (BSAP), and an excellent correlation was found with the bone-strontium content. In females, the placebo-treated group showed a decrease in BSAP of 53%, whereas the three strontium malonate-treated groups showed an increase of 60, 276, and 278% for the groups treated with 300, 1000, and 3000 mg kg−1 day−1, respectively. For males the corresponding values were −44%, +142%, +194%, and +247% increases in BSAP in the placebo, 300, 1000, and 3000 mg kg−1 day−1 groups respectively.
Similar content being viewed by others
References
Shorr E, Carter A (1952) Bull Hosp Joint Dis 13:59–66
McCaslin FE, Janes JM (1959) Proc Staff Meetings of the Mayo Clinic 34:329–334
Sorbera LA, Castaner J, Leeson PA, Bayes M (2003) Drug Future 28:328–335
Nielsen SP (2004) Bone 35:583–588
Marie PJ (2003) Osteoporos Int 14(Suppl 3):S9–S12
Canalis E, Hott M, Deloffre P, Tsouderos Y, Marie PJ (1996) Bone 18:517–523
Marie PJ, Ammann P, Boivin G, Rey C (2001) Calc Tiss Inter 69:121–129
Jensen J-EB, Stang H, Kringsholm B (1997) Bone 20(S4):104–108
Boivin G, Meunier PJ (2003) Osteopor Inter 14:S19–S24
Dahl SG, Allain P, Marie PJ, Mauras Y, Boivin G, Ammann P, Tsouderos Y, Delmas PD, Christiansen C (2001) Bone 28:446–453
Briggman B, Oskarsson A (1977) Acta Crystallogr B 33:1900–1906
Christensen A (2005) Strontium malonate–4 week toxicity study in dogs with toxicokinetics, Scantox Study report 59147
Helsby CA (1974) Anal Chim Acta 69:259–265
Burguera M, Burguera JL, Di Bernardo ML, Alarcon OM, Nieto E, Salinas JR, Burguera E (2002) Tr Elem Electr 19:143–151
Scancar J, Milacic R, Benedik M, Bukovec P (2000) Clin Chim Acta 293:187–197
Burguera M, Burguera JL, Rondon C, Di Bernardo ML, Gallignani M, Nieto E, Salinas J (1999) Spectrochim Acta B 54:805–818
D’haese PC, VanLandeghem GF, Lamberts LV, Bekaert VA, Schrooten I, Debroe ME (1997) Clin Chem 43:121–128
Burguera M, Burguera JL, Di Bernardo ML, Rondon C, Carrero P, Nieto E, Salinas R, Burguera E (1999) Quim Anal 18:305–312
Roberts NB, Walsh HPJ, Klenerman L, Kelly SA, Helliwell TR (1996) J Anal At Spectrosc 11:133–138
Outridge PM, Hughes RJ, Evans RD (1996) At Spectrosc 17:1–8
Kang D, Amarasiriwardena D, Goodman AH (2004) Anal Bioanal Chem 378:1608–1615
Gy PM (1998) Sampling for analytical purposes. Wiley, Chichester
Hasegawa T, Matsuura H, Inagaki K, Haraguchi H (2003) Anal Sci 19:147–150
Hsiung CS, Andrade JD, Costa R, Ash KO (1997) Clin Chem 43:2303–2311
Goulle JP, Mahieu L, Castermant J, Neveu N, Bonneau L, Laine G, Bouige D, Lacroix C (2005) For Sci Inter 153:39–44
Vezzoli G, Baragetti I, Zerbi S, Caumo A, Soldati L, Bellinzoni P, Centemero A, Rubinacci A, Moro G, Bianchi G (1998) Clin Chem 44:586–590
Olsen KJ (2005) Four week toxicity study in dogs with toxicokinetics, end of text listings, tables, figures and statistical output, Study NBS T07 GE
Olsen KJ (2005) Four week toxicity study in dogs with toxicokinetics, Statistical report of toxicokinetic data, Study NBS T07 GE
Marie PJ (2006) Bone 38:S10–S14
Boivin G, Deloffre P, Perrat B, Panczer G, Boudeulle M, Mauras Y, Allain P, Tsouderos Y, Meunier PJ (1996) J Bone Miner Res 11:1302–1311
Cabrera WE, Schrooten I, De Broe ME, D’Haese PC (1999) J Bone Miner Res 14:661–668
Gawlik D, Behne D, Bratter P, Gatschke W, Gessner H, Kraft D (1982) J Clin Chem Clin Biochem 20:499–507
Danzer K, Currie LA (1998) Pure Appl Chem 70:993–1014
Inczedy J, Lengyel T, Ure AM (1998) International Union of Pure and Applied Chemistry, Compendium of analytical nomenclature, online ed, Blackwell Science; http://www.iupac.org/publications/books/author/inczedy.html
Brüggemann L, Morgenstern P, Wennrich R (2005) Accred Qual Assur 10:344–351
Miller JN, Miller JC (2005) Statistics and chemometrics for analytical chemistry, 5th edn. Pearson, Edinburgh
Analytical Methods Committee (AMC) (2002), Technical Brief no. 10. Fitting a linear functional relationship to data with error on both variables, Royal Society of Chemistry, Cambridge; http://www.rsc.org/Membership/Networking/InterestGroups/Analytical/AMC/TechnicalBriefs.asp
Miller JN (1991) Analyst 116:3–14
Ellison SLR, Rösslein M, Williams A (eds) (2000) Eurachem/CITAC Guide, Quantifying uncertainty in analytical measurement, 2nd edn
Cuadros-Rodriguez L, Gamiz-Cracia L, Almansa-Lopez E, Laso-Sanchez J (2001) Trends Anal Chem 20:195–206
International Organization for Standardization (1995) Guide to the expression of uncertainty in measurement (GUM). Geneva, Switzerland
Acknowledgements
Financial support from the Director Per Henriksen’s Foundation, the Idella Foundation, and from the Carlsberg Foundation is gratefully acknowledged.
Many thanks are due to Flemming E. Hansen for his expert technical assistance in operation of the Elan 5000 ICP–MS.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Raffalt, A.C., Andersen, J.E.T. & Christgau, S. Application of inductively coupled plasma–mass spectrometry (ICP–MS) and quality assurance to study the incorporation of strontium into bone, bone marrow, and teeth of dogs after one month of treatment with strontium malonate. Anal Bioanal Chem 391, 2199–2207 (2008). https://doi.org/10.1007/s00216-008-2171-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-008-2171-0