Abstract
A variety of techniques exist that provide chemical information in the form of a spatially resolved image: electron microprobe analysis, nuclear microprobe analysis, synchrotron radiation microprobe analysis, secondary ion mass spectrometry, and confocal fluorescence microscopy. Linear (LINAC) and circular (synchrotrons) particle accelerators have been constructed worldwide to provide to the scientific community unprecedented analytical performances. Now, these facilities match at least one of the three analytical features required for the biological field: (1) a sufficient spatial resolution for single cell (< 1 μm) or tissue (<1 mm) analyses, (2) a temporal resolution to follow molecular dynamics, and (3) a sensitivity in the micromolar to nanomolar range, thus allowing true investigations on biological dynamics. Third-generation synchrotrons now offer the opportunity of bioanalytical measurements at nanometer resolutions with incredible sensitivity. Linear accelerators are more specialized in their physical features but may exceed synchrotron performances. All these techniques have become irreplaceable tools for developing knowledge in biology. This review highlights the pros and cons of the most popular techniques that have been implemented on accelerator-based sources to address analytical issues on biological specimens.
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References
Kocsis M, Snigirev A (2004) Nucl Instrum Methods Phys A 525:79–84
Mondelaers W, Cauwels P, Masschaele B, Dierick M, Lahorte P, Jolie J, Baechler S, Materna T (2000) XX international linac conference, Monterey, California THC 16:890–892
Incerti S, Zhang Q, Andersson F, Moretto P, Grime GW, Merchant MJ, Nguyen DT, Habchi C, Pouthier T, Seznec H (2007) Nucl Instrum Methods Phys B 260:20–27
Gorelick S, Rahkila P, Sagari AR, Sajavaara T, Cheng S, Karlsson LB, van Kand JA, Whitlow HJ (2007) Nucl Instrum Methods Phys B 260:130–135
Gilbert B, Margaritondo G, Mercanti D, Casalbore P, De Stasio G (2001) J Alloy Compd 328:8–13
Fahrni CJ (2007) Curr Opin Chem Biol 11:121–127
Petibois C, Gionnet K, Goncalves M, Perromat A, Moenner M, Déléris G (2006) Analyst 131:640–647
Ortega R, Biston M-C, Devès G, Bohic S, Carmona A (2005) Nucl Instrum Methods Phys B 231:321–325
Aburaya JH, Added N, Tabacniks MH, de Almeida Rizzutto M, Dupret M, Barbosa L (2006) Nucl Instrum Methods Phys B 249:792–795
Rokita E, Chevallier P, Mutsaers PHA, Stopa M, Taton G, de Voigt MJA (2000) Nucl Instrum Methods Phys B 161–163:887–893
Ortega R, Deves G, Moretto P (2001) Nucl Instrum Methods Phys B 181:475–479
Minqin R, van Kan JA, Bettiol AA, Daina L, Gek CY, Huat BB, Whitlow HJ, Osipowicz T, Watt F (2007) Nucl Instrum Methods Phys B 260:124–129
Aratono M, Kashimoto K, Matsuda T, Muroi S, Takata Y, Ikeda N, Takiue T, Tanida H, Watanabe I (2005) Langmuir 21:7398–7404
Farquharson MJ, Geraki K, Falkenberg G, Leek R, Harris A (2007) Appl Radiat Isot 65:183–188
Rao DV, Yuasa T, Akatsuka T, Tromba G, Hasan MZ, Takeda T, Devaraj B (2006) Rad Meas 41:177–182
Ward NL, Haninec AL, Van Slyke P, Sled JG, Sturk C, Henkelman RM, Wanless IR, Dumont DJ (2004) Am J Pathol 165:889–899
Petibois C, Cazorla G, Cassaigne A, Deleris G (2001) Clin Chem 47:730–738
Petibois C, Drogat B, Bikfalvi A, Deleris G, Moenner M (2007) FEBS Lett 581:5469–5474
Petibois C, Gouspillou G, Wehbe K, Delage JP, Deleris G (2006) Anal Bioanal Chem 386:1961–1966
Petibois C, Déléris G (2006) Trends Biotechnol 24:455–462
Cestelli-Guidi M, Piccinini M, Marcelli A, Nucara A, Calvani P, Burattini E (2005) J Opt Soc Am 22:2810–2817
Jamin N, Dumas P, Moncuit J, Fridman WH, Teillaud JL, Carr GL, Williams GP (1998) Proc Natl Acad Sci USA 95:4837–4840
Burghardt AJ, Wang Y, Elalieh H, Thibault X, Bikle D, Peyrin F, Majumdar S (2007) Bone 40:160–168
Miller LM, Tague TJ Jr (2002) Vibrational Spectrosc 28:159–165
Brucherseifer M, Nagel M, Haring PB, Kurz H (2000) Appl Phys Lett 77:4049–4051
Grosse E (2002) Phys Med Biol 47:3755–3760
Edwards GS, Austin RH, Carroll FE, Copeland ML, Couprie ME, Gabella WE, Haglund RF, Hooper BA, Hutson MS, Jansen ED, Joos KM, Kiehart DP, Lindau I, Miao J, Pratisto HS, Shen JH, Tokutake Y, van der Meer AFG, Xie A (2003) Rev Sci Instrum 74:3207–3245
Carroll FE, Mendenhall MH, Traeger RH, Brau C, Waters JW (2003) Am J Radiat 181:1197–1202
Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J (2001) Nat Struct Biol 8:689–694
Hajdu J, Neutze R, Sjogren T, Edman K, Szoke A, Wilmouth RC, Wilmot CM (2000) Nat Struct Biol 7:1006–1012
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Petibois, C., Cestelli Guidi, M. Bioimaging of cells and tissues using accelerator-based sources. Anal Bioanal Chem 391, 1599–1608 (2008). https://doi.org/10.1007/s00216-008-2157-y
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DOI: https://doi.org/10.1007/s00216-008-2157-y