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Circular dichroism thermal lens microscope in the UV wavelength region (UV-CD-TLM) for chiral analysis on a microchip

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Abstract

We have developed a circular-dichroism thermal lens microscope for UV wavelengths (UV-CD-TLM), for the first time, to realize sensitive chiral analysis on a microchip. Quasi-continuous-wave phase modulation of a pulsed UV laser was used to generate left-circularly polarized light and right-circularly polarized light and to detect the generated TL signal amplitude and phase with a lock-in amplifier. The amplitude and phase were used to determine the concentration and chirality, respectively, of a sample. The basic principle of UV-CD-TLM for chiral analysis on a microchip was verified by measuring aqueous solutions of optically active camphorsulfonic acids (CSA). Lower limits of detection (LOD) were calculated at S/N = 2 and were 8.7 × 10−4 mol L−1 (ΔA = 5.2 × 10−6 Abs.) for (+)-CSA and 8.4 × 10−4 mol L−1 (ΔA = 5.0 × 10−6 Abs.) for (−)-CSA. In terms of number of molecules, LODs for UV-CD-TLM were calculated to be 8.7 fmol and 8.4 fmol, respectively. This is at least three orders of magnitude lower than previously obtained. The applicability of UV-CD-TLM for chiral analysis on a microchip was verified.

Sensitive chiral analysis by thermal lens microscope (TLM)

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Acknowledgement

We would like to thank Dr Haeng-Boo Kim of Ibaraki University for valuable discussion and suggestions.

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Authors and Affiliations

  1. Microchemical Group, Kanagawa Academy of Science and Technology, 3-2-1 Sakado, Takatsu, Kawasaki, Kanagawa, 213-0012, Japan

    Kazuma Mawatari & Takehiko Kitamori

  2. Department of Applied Chemistry, School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Shun Kubota & Takehiko Kitamori

Authors
  1. Kazuma Mawatari
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  2. Shun Kubota
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  3. Takehiko Kitamori
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Correspondence to Takehiko Kitamori.

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Mawatari, K., Kubota, S. & Kitamori, T. Circular dichroism thermal lens microscope in the UV wavelength region (UV-CD-TLM) for chiral analysis on a microchip. Anal Bioanal Chem 391, 2521–2526 (2008). https://doi.org/10.1007/s00216-008-2006-z

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  • DOI: https://doi.org/10.1007/s00216-008-2006-z

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