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Contribution of multi-nuclear solid state NMR to the characterization of the Thalassiosira pseudonana diatom cell wall

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Abstract

A major issue in the study of biosilicification processes is the harsh chemical conditions required for silica dissolution, which often lead to denaturation of the associated bio-organic matter. In order to demonstrate the potential of solid state NMR for investigating silicified materials of natural origin, this technique was applied to isotopically enriched Thalassiosira pseudonana diatom cells. 29Si, 1H,31P, 13C and 15N solid state NMR studies were performed on whole cells, SDS-extracted and H2O2-cleaned silica shells. Cross-polarization techniques were useful for identifying the presence of mobile and rigid molecules, allowing loosely bound and silica-entrapped species to be discriminated. Successive cleaning procedures efficiently eliminated weakly associated organic matter. The H2O2-cleaned silica shell still contained carbohydrates (mainly chitin) and proteins as well as lipids. This suggests that the role of lipids in diatom shell formation may have been underestimated so far, demonstrating the potential of solid state NMR for studying composite biomaterials.

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Acknowledgments

The authors are very grateful to Dr Thierry Azaïs (CMCP) for fruitful discussions. B. Tesson and V. Martin-Jézéquel are funded by the Region Pays de Loire, the University of Nantes, the Centre National de la Recherche Scientifique (CNRS) and the EC Sixth Framework Programme “Diatomics” (LSHG-CT-2004-512035).

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Authors and Affiliations

  1. EA 2663, Faculté des Sciences, Université de Nantes, 2 rue de la Houssinière, BP 92208, 44322, Nantes, France

    Benoit Tesson & Véronique Martin-Jézéquel

  2. UPMC Univ Paris 6, UMR 7574, Chimie de la Matière Condensée de Paris, 75005, Paris, France

    Sylvie Masse, Guillaume Laurent, Jocelyne Maquet, Jacques Livage & Thibaud Coradin

  3. CNRS, UMR 7574, Chimie de la Matière Condensée de Paris, 75005, Paris, France

    Sylvie Masse, Guillaume Laurent, Jocelyne Maquet & Thibaud Coradin

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  1. Benoit Tesson
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  2. Sylvie Masse
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  3. Guillaume Laurent
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  4. Jocelyne Maquet
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  5. Jacques Livage
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  6. Véronique Martin-Jézéquel
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  7. Thibaud Coradin
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Corresponding author

Correspondence to Thibaud Coradin.

Electronic supplementary material

A list of 13C MAS NMR resonance chemical shifts and proposed attributions for the H2O2-cleaned T. pseudonana sample is provided in Table S1.

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Tesson, B., Masse, S., Laurent, G. et al. Contribution of multi-nuclear solid state NMR to the characterization of the Thalassiosira pseudonana diatom cell wall. Anal Bioanal Chem 390, 1889–1898 (2008). https://doi.org/10.1007/s00216-008-1908-0

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  • DOI: https://doi.org/10.1007/s00216-008-1908-0

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