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Collision-induced reporter fragmentations for identification of covalently modified peptides

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Abstract

Collision-induced reporter fragmentations of the currently most important covalent peptide modifications as detected by tandem mass spectrometry are summarized. These fragmentations comprise the formation of reporter ions, which are preferentially immonium ions, immonium ion-derived fragments or side chain fragments. In addition, the reporter neutral loss reactions for covalently modified amino acid residues are summarized. For each individual covalent modification which can be recognized by a reporter fragmentation, the accurate mass shift and the gross formula shift of the modified amino acid residue are given. The same set of data is provided for the reporter fragmentations. Finally, an extensive accurate mass and gross formula list is presented as supplementary material, describing mostly regular and modified y1 and dipeptide a and b ions, which are helpful for identification of the peptide ends of covalently modified peptides.

When modified peptides are fragmented by collision-induced dissociation in a tandem mass spectrometer, the modification is either lost as part of a charged fragment, so that a reporter ion for the modification is generated or it is lost as part of a neutral fragment, so that a modification-specific reporter neutral loss is observed in the fragment ion spectrum.

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Acknowledgements

We are indebted to D. Kübler, W.E. Hull and R. Pipkorn for valuable support.

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Authors and Affiliations

  1. Molecular Structure Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

    Chien-Wen Hung & Wolf D. Lehmann

  2. Core Facility Proteomics, Center for Systems Biology (ZBSA), Freiburg, Germany

    Andreas Schlosser

  3. Department of Biochemistry, University of Washington, Seattle, WA, USA

    Junhua Wei

Authors
  1. Chien-Wen Hung
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  2. Andreas Schlosser
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  3. Junhua Wei
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  4. Wolf D. Lehmann
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Corresponding author

Correspondence to Wolf D. Lehmann.

Additional information

Chien-Wen Hung and Andreas Schlosser contributed equally to this work.

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Collision-induced Reporter Fragmentations for Identification of Covalently Modified Peptides (PDF 1 457 kb)

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Hung, CW., Schlosser, A., Wei, J. et al. Collision-induced reporter fragmentations for identification of covalently modified peptides. Anal Bioanal Chem 389, 1003–1016 (2007). https://doi.org/10.1007/s00216-007-1449-y

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  • DOI: https://doi.org/10.1007/s00216-007-1449-y

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