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A simple and rapid method for simultaneous determination of benzoic and sorbic acids in food using in-tube solid-phase microextraction coupled with high-performance liquid chromatography

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Abstract

A simple, rapid and sensitive on-line method for the simultaneous determination of benzoic and sorbic acids in food was developed by coupling in-tube solid-phase microextraction (SPME) to high-performance liquid chromatography (HPLC) with UV detection. The diethylamine-modified poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic capillary selected as the extraction medium exhibited a high extraction capability towards benzoic and sorbic acids. To obtain optimum extraction performance, several in-tube SPME parameters were investigated, including pH value, inorganic salt, and the organic solvent content of the sample matrix. After simple dilution with 0.02 mol/L phosphate solution (pH 4.0), carbonated drink, juice drink, sauce and jam samples could be directly injected for extraction. For succade samples, a small amount of acetonitrile was required to extract analytes prior to dilution and subsequent extraction. The linearity of the method was investigated over a concentration range of 5–20000 ng/mL for both analytes, and the correlation coefficients (R 2 values) were higher than 0.999. The detection limits for benzoic and sorbic acids were 1.2 and 0.9 ng/mL, respectively. The method reproducibility was tested by evaluating the intra- and interday precisions; relative standard deviations of less than 4.4 and 9.9%, respectively, were obtained. Recoveries of compounds from spiked food samples ranged from 84.4 to 106%. The developed method was shown to be suitable for the routine monitoring of benzoic and sorbic acids in various types of food samples.

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Acknowledgments

This work is partly supported by grants from the National Science Fund for Distinguished Young Scholars (No.20625516) and the Program for New Century Excellent Talents in University (NCET-05-0616).

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Authors and Affiliations

  1. Department of Chemistry, Wuhan University, Wuhan, 430072, China

    Yi Wen, Ying Wang & Yu-Qi Feng

  2. Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, 430072, China

    Yu-Qi Feng

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  1. Yi Wen
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  2. Ying Wang
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  3. Yu-Qi Feng
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Correspondence to Yu-Qi Feng.

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Wen, Y., Wang, Y. & Feng, YQ. A simple and rapid method for simultaneous determination of benzoic and sorbic acids in food using in-tube solid-phase microextraction coupled with high-performance liquid chromatography. Anal Bioanal Chem 388, 1779–1787 (2007). https://doi.org/10.1007/s00216-007-1395-8

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  • DOI: https://doi.org/10.1007/s00216-007-1395-8

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