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Polycation coating poly(dimethylsiloxane) capillary electrophoresis microchip for rapid separation of ascorbic acid and uric acid

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Abstract

A novel method for rapid separation and determination of ascorbic acid and uric acid has been developed with a polycation-modified poly(dimethylsiloxane) (PDMS) microchip under a negative-separation electric field. Just by flushing the microchip with aqueous solutions of the polycations, poly(allylamine) hydrochloride, poly(diallyldimethylammonium chloride) or chitosan could be stably coated on the PDMS microchannel surface, which resulted in a reversed electroosmotic flow and thus the rapid and efficient separation of the two substrates. Factors influencing the separation, including polycation category, buffer solution, detection potential and separation voltage, were investigated and optimized. The cheapness, rapid analysis speed and the successful analysis of human urine make this microsystem attractive for application in clinics.

The electropherograms of 100 μ/mL AA and UA in (1) PAH, (2) PDDA, (3) Chitosan modified PDMS microchannels and native PDMS microchip (4).

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (nos. 20475025, 20575029, 20635002), the National Natural Science Funds for Creative Research Groups (20521503) and the key project of the Science and Technology Research of MOE (106080).

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Authors and Affiliations

  1. The Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China

    Q. L. Zhang, J. J. Xu, H. Z. Lian, X. Y. Li & H. Y. Chen

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  1. Q. L. Zhang
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  2. J. J. Xu
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  3. H. Z. Lian
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  4. X. Y. Li
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  5. H. Y. Chen
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Correspondence to H. Y. Chen.

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Zhang, Q.L., Xu, J.J., Lian, H.Z. et al. Polycation coating poly(dimethylsiloxane) capillary electrophoresis microchip for rapid separation of ascorbic acid and uric acid. Anal Bioanal Chem 387, 2699–2704 (2007). https://doi.org/10.1007/s00216-007-1173-7

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  • DOI: https://doi.org/10.1007/s00216-007-1173-7

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