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Analysis of the hierarchy of quorum-sensing regulation in Pseudomonas aeruginosa

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Abstract

Quorum-sensing in Pseudomonas aeruginosa is known to regulate several aspects of pathogenesis, including virulence factor production, biofilm development, and antimicrobial resistance. Recent high-throughput analysis has revealed the existence of several layers of regulation within the QS-circuit. To address this complexity, mutations in genes encoding known or putative transcriptional regulators that were also identified as being regulated by the las and/or rhl QS systems were screened for their contribution in mediating several phenotypes, for example motility, secreted virulence products, and pathogenic capacity in a lettuce leaf model. These studies have further elucidated the potential contribution to virulence of these genes within the QS regulon.

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Acknowledgements

The authors thank Dr Kristin Picardo for assistance with the lettuce leaf model of infection and Jane Malone for technical assistance with autoinducer extractions. This work was supported by a grant from the National Institutes of Health to B.H.I (NIHR37AI37713). V.E.W. is supported by an NIH training grant (5T32AI07285).

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Authors and Affiliations

  1. Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, Box 672, Rochester, NY, 14642, USA

    Victoria E. Wagner, Luen-Luen Li, Vincent M. Isabella & Barbara H. Iglewski

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  1. Victoria E. Wagner
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  2. Luen-Luen Li
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  3. Vincent M. Isabella
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  4. Barbara H. Iglewski
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Correspondence to Barbara H. Iglewski.

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Wagner, V.E., Li, LL., Isabella, V.M. et al. Analysis of the hierarchy of quorum-sensing regulation in Pseudomonas aeruginosa . Anal Bioanal Chem 387, 469–479 (2007). https://doi.org/10.1007/s00216-006-0964-6

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  • DOI: https://doi.org/10.1007/s00216-006-0964-6

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