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Fluorescence characterization of metal ion–humic acid interactions in soils amended with composted municipal solid wastes

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Abstract

Fluorescence spectroscopy has been used to probe the structural properties and Cu(II), Zn(II), Cd(II), and Pb(II)-binding behavior of humic acid (HA)-like fractions isolated from a municipal solid waste compost (MSWC) and HAs from unamended and MSWC-amended soils. The main feature of the fluorescence spectra, in the form of emission-excitation matrix (EEM) plots, was a broad peak with the maximum centered at an excitation/emission wavelength pair that was much shorter (340/437 nm) for MSWC-HA than for unamended and MSWC-amended soil HAs (455/513 and 455/512 nm, respectively). Fluorescence intensity for MSWC-amended soil HA was less than that for unamended soil HA. These results were indicative of more aromatic ring polycondensation and humification of soil HAs, and of partial incorporation of simple and low-humified components of MSWC-HA into native soil HA, as a result of MSWC amendment. Titrations of HAs with Cu(II), Zn(II), Cd(II), and Pb(II) ions at pH 6 and ionic strength 0.1 mol L−1 resulted in a marked decrease of the fluorescence intensities of untreated HAs. By successfully fitting a single-site fluorescence-quenching model to titration data, the metal ion complexing capacities of each HA and the stability constants of metal ion-HA complexes were obtained. The binding capacities and stability constants of MSWC-HA were smaller than those of the unamended soil HA. Application of MSWC to soil slightly reduced the metal-ion-binding capacities and affinities of soil HAs.

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Acknowledgements

The authors are grateful to Professor C.E. Clapp, Department of Soil, Water and Climate, University of Minnesota, St Paul, MN, USA, for providing the MSWC and soil samples used in this work. C. Plaza is a recipient of a Ramón y Cajal contract funded by the Spanish Ministry of Education and Science.

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Authors and Affiliations

  1. Centro de Ciencias Medioambientales, Consejo Superior de Investigaciones Científicas, Serrano 115 dpdo., 28006, Madrid, Spain

    César Plaza & Alfredo Polo

  2. Dipartimento di Biologia e Chimica Agroforestale ed Ambientale, University of Bari, Via Amendola 165/A, 70126, Bari, Italy

    Gennaro Brunetti & Nicola Senesi

Authors
  1. César Plaza
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  2. Gennaro Brunetti
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  3. Nicola Senesi
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  4. Alfredo Polo
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Correspondence to César Plaza.

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Plaza, C., Brunetti, G., Senesi, N. et al. Fluorescence characterization of metal ion–humic acid interactions in soils amended with composted municipal solid wastes. Anal Bioanal Chem 386, 2133–2140 (2006). https://doi.org/10.1007/s00216-006-0844-0

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  • DOI: https://doi.org/10.1007/s00216-006-0844-0

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