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Study of normal colorectal tissue by FT-Raman spectroscopy

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Abstract

FT-Raman spectroscopy was employed to study normal human colorectal tissues in vitro with the aim of evaluating the spectral differences of the complex colon mucous in order to establish a characteristic Raman spectrum. The samples were collected from 39 patients, providing 144 spectra for the statistical analysis. The results enable one to estabilish three well-defined spectroscopic groups of non-altered coloretal tissues that were consistently checked by statistical (clustering) and biological (histopathology) analyses: group 1 is represented by samples with the presence of epithelial layer, connective tissue papillae, and smooth muscle tissue; group 2 comprises tissues with epithelial layer and connective tissue papillae; group 3 presented mostly fatty and slack conjunctive tissue. The study reveals the existence of an intrinsic spectral variability for each patient that must be considered when sampling tissues fragments to build a spectral database. This is the first step for future studies and applications of Raman spectroscopy to optical biopsy and diagnosis of colorectal cancer.

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Acknowledgments

The authors thank the Colonoscopy Service of the Portuguese Charity Hospital of São José dos Rio Preto, São Paulo, the Coloproctology Department of the Medicine Faculty of São José do Rio Preto (FUNFARME), where surgeries were performed, and the Vivalle Hospital of São José dos Campos, SP, for the samples supplement. This work was supported by CNPq (project 401018/2005-9) and FAPESP (project 01/14384-8).

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Authors and Affiliations

  1. Laboratory of Biomedical Vibrational Spectroscopy, Institute of Research and Development (IP&D), Universidade do Vale do Paraiba (UniVap), Av. Shishima Hifumi, 2911, 12244-000, São José dos Campos, SP, Brazil

    P. O. Andrade, R. A. Bitar, K. Yassoyama, H. Martinho, A. M. E. Santo & A. A. Martin

  2. Vivalle Hospital, Av. Lineu de Moura 995, 12244-380, São José dos Campos, SP, Brazil

    P. M. Bruno

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  1. P. O. Andrade
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  2. R. A. Bitar
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  3. K. Yassoyama
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  4. H. Martinho
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  5. A. M. E. Santo
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  6. P. M. Bruno
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  7. A. A. Martin
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Correspondence to A. A. Martin.

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Andrade, P.O., Bitar, R.A., Yassoyama, K. et al. Study of normal colorectal tissue by FT-Raman spectroscopy. Anal Bioanal Chem 387, 1643–1648 (2007). https://doi.org/10.1007/s00216-006-0819-1

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  • DOI: https://doi.org/10.1007/s00216-006-0819-1

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