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Development of RNR3- and RAD54-GUS reporters for testing genotoxicity in Saccharomyces cerevisiae

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Abstract

S. cerevisiae RNR3 and RAD54 gene transcription becomes strongly activated upon DNA damage. This property was used to construct yeast strains in which DNA damage can be monitored by a very sensitive fluorogenic assay in a convenient 96-well microtiter plate format. These strains carried stably integrated fusions of RNR3 or RAD54 promoters to the E. coli β-glucuronidase GUS gene. GUS activity was measured by fluorogenic detection, a method that greatly increases the precision and sensitivity of the assay. Detection levels were similar to those of real-time quantitative PCR methods and close to the limits of biological response. The two reporters differed in terms of fold-induction, activation kinetics, sensitivity and specificity upon exposure to a variety of genotoxic compounds. While RNR3-GUS showed the fastest response, RAD54-GUS showed the highest sensitivity: similar to previous reported sensitivities for bacterial and eukaryotic genotoxic detection systems. These reporter strains may complement current genotoxicity tests, but they also have the advantages of higher flexibility, requirement for shorter incubation times, and the capability of being fully automated. In addition, the intrinsic features of the system facilitate its easy improvement by genetic manipulating the yeast strain or by introducing mammalian metabolizing enzymes.

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Acknowlegments

This work has been supported by the Spanish Ministry for Science and Technology (BIO2005-00840 and GEN2001-4707-C08-08). The contribution of the Centre de Referència en Biotecnologia de la Generalitat de Catalunya is also acknowledged.

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  1. Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Jordi Girona, 18, 08034, Barcelona, Spain

    Susanna Boronat & Benjamin Piña

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  1. Susanna Boronat
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  2. Benjamin Piña
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Correspondence to Benjamin Piña.

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Boronat, S., Piña, B. Development of RNR3- and RAD54-GUS reporters for testing genotoxicity in Saccharomyces cerevisiae . Anal Bioanal Chem 386, 1625–1632 (2006). https://doi.org/10.1007/s00216-006-0751-4

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  • DOI: https://doi.org/10.1007/s00216-006-0751-4

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