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Analysis of carbamazepine and its active metabolite, carbamazepine-10,11-epoxide, in human plasma using high-performance liquid chromatography

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Abstract

A sensitive method based on high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was developed for the determination of carbamazepine (CBZ) and one of its active metabolites, carbamazepine-10,11-epoxide (CBZ-E) in human plasma. CBZ, CBZ-E and the internal standard (IS) 10,11-dihydrocarbamazepine were extracted from human plasma into methyl tert-butyl ether. CBZ, CBZ-E and the IS were successfully separated on an RP C18 column with a mobile phase of acetonitrile:methanol:water (18:19:63, v/v/v) and monitored via UV detection at 210 nm. The calibration curves were linear over the concentration ranges of 0.01–10 μg/mL for CBZ and 0.005–5 μg/mL for CBZ-E in human plasma, respectively. The method displayed excellent sensitivity, precision and accuracy, and was successfully applied to the quantification of CBZ and CBZ-E in human plasma after oral administration of a single 200 mg CBZ CR tablet. This method is suitable for bioequivalence studies following single doses given to healthy volunteers.

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Acknowledgements

This study was supported by Whanin Pharm. Co. (Seoul, Republic of Korea) through the Research Institute of Pharmaceutical Sciences, Seoul National University.

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Authors and Affiliations

  1. Laboratory of Excellency for Drug and Gene Delivery, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul, 151-742, South Korea

    Eun kyung Oh, Eunmi Ban & Chong-Kook Kim

  2. Hanmi Pharm Co., Ltd., 893-5, Hajeo-ri, Paltan-myeon, Hwasung-si, Gyeonggi-do, South Korea

    Jong Soo Woo

Authors
  1. Eun kyung Oh
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  2. Eunmi Ban
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  3. Jong Soo Woo
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  4. Chong-Kook Kim
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Correspondence to Chong-Kook Kim.

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Oh, E.k., Ban, E., Woo, J.S. et al. Analysis of carbamazepine and its active metabolite, carbamazepine-10,11-epoxide, in human plasma using high-performance liquid chromatography. Anal Bioanal Chem 386, 1931–1936 (2006). https://doi.org/10.1007/s00216-006-0724-7

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  • DOI: https://doi.org/10.1007/s00216-006-0724-7

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