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Improved online coupling of planar chromatography with electrospray mass spectrometry: extraction of zones from glass plates

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Abstract

A plunger-based extraction device for HPTLC/MS coupling, which was originally designed for extraction on TLC aluminum foils, was enhanced. The threefold modifications enabled extraction of analytes from glass-backed HPTLC/TLC plates after separation. A buffering of the plunger reduced the occurrence of leakage. The involvement of a torque screwdriver for the fixation resulted in a reproducible contact pressure and avoided breaking the glass plates. The employment of this device was also extended to plates with a layer thickness of 100 μm by reducing the height of the plunger’s cutting edge. Repeatability of the extraction from glass-backed plates, linearity of the signal obtained, and detection capability were shown to be comparable to the original device, which was only usable with aluminum foils. The influence of the elution solvent on the intensity of the MS signal was demonstrated to be a compromise between high elution power of the solvent and good solubility of the analyte in the elution solvent. The extraction device was employed for plates from different lots and for plates with different stationary phases thereby proving its general applicability in planar chromatography.

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References

  1. Fanibanda T, Milnes J, Gormally J (1994) Int J Mass Spectrom Ion Processes 140:127–130

    Article  CAS  Google Scholar 

  2. Gusev AI (2000) Fresenius J Anal Chem 366:691–700

    Article  CAS  Google Scholar 

  3. Crecelius A, Clench MR, Richards DS (2004) PharmaGenomics 4:36–38, 40, 42

    CAS  Google Scholar 

  4. Salo PK, Salomies H, Harju K, Ketola RA, Kotiaho T, Yli-Kauhaluoma J, Kostiainen R (2005) J Am Soc Mass Spectrom 16:906–915

    Article  CAS  Google Scholar 

  5. Costello CE, Guittard JG, Hronowski XL, Costello CE (1999) Rapid Commun Mass Spectrom 13:1838–1849

    Article  Google Scholar 

  6. Dreisewerd K, Kölbl S, Peter-Katalinic J, Berkenkamp J, Pohlentz G (2006) J Am Soc Mass Spectrom 17:139–150

    Article  CAS  Google Scholar 

  7. Oriňák A, Vering G, Arlinghaus HF, Andersson JT, Halas L, Oriňáková R (2005) J Planar Chromatogr Modern TLC 18:44–50

    Article  Google Scholar 

  8. Oka H, Ikai Y, Hayakawa J, Harada K, Masuda K, Suzuki M, Himei R, Horie M, Nakazawa H (1993) J Food Hygienic Soc Japan 34:517–523

    CAS  Google Scholar 

  9. Van Berkel GJ, Ford MJ, Deibel M.A. (2005) Anal Chem 77:1207–1215

    Article  Google Scholar 

  10. Cody RB, Laramee JA, Durst HD (2005) Anal Chem 77:2202–2297

    Article  Google Scholar 

  11. Morlock G, Schwack W (2006) Anal Bioanal Chem 385:586–595

    Article  CAS  Google Scholar 

  12. Anderson RM, Busch KL (1998) J Planar Chromatogr Modern TLC 11:336–341

    CAS  Google Scholar 

  13. Kertesz V, Van Berkel GJ, Ford MJ (2005) Anal Chem 77:7183–7189

    Article  CAS  Google Scholar 

  14. Prosek M, Milivojevic L, Krizman M (2004) J Planar Chromatogr Modern TLC 6:420–423

    Article  Google Scholar 

  15. Luftmann H (2004) Anal Bioanal Chem 378:964–968

    Article  CAS  Google Scholar 

  16. Morlock G, Nedele A, Schwack W (2005) Euro Food Chem XIII - Proceedings 2:513–516

    Google Scholar 

  17. Jautz U, Morlock G (2006) J Chromatogr A (in press)

  18. Giachetti C, Assandri A, Zanolo G (1991) J Chromatogr 585:111–115

    Article  CAS  Google Scholar 

  19. Herbert P, Santos L, Bastos M, Barros P, Alves A (2002) Food Chem Toxicol 67:1616–1620

    CAS  Google Scholar 

  20. Morlock G (2006) (in preparation)

Download references

Acknowledgements

The authors thank Professor Dr. Wolfgang Schwack, University of Hohenheim, for the excellent working conditions at the Institute of Food Chemistry. Special thanks go to Dr. Heinz-Emil Hauck, Merck, Darmstadt, Germany, for supply of plate material; to Dr. Luftmann, University of Münster, Germany, for providing the ChromeXtractor device; and to Dr. Konstantinos Natsias, CAMAG, Berlin, Germany, for support regarding equipment. Great thank goes to Landesstiftung Baden-Württemberg for financial support (project no. P-LS-E2/25).

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Authors and Affiliations

  1. Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, 70599, Stuttgart, Germany

    Alexander Alpmann & Gertrud Morlock

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  1. Alexander Alpmann
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  2. Gertrud Morlock
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Correspondence to Gertrud Morlock.

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Alpmann, A., Morlock, G. Improved online coupling of planar chromatography with electrospray mass spectrometry: extraction of zones from glass plates. Anal Bioanal Chem 386, 1543–1551 (2006). https://doi.org/10.1007/s00216-006-0692-y

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  • DOI: https://doi.org/10.1007/s00216-006-0692-y

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