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Quantification of abscisic acid in grapevine leaf (Vitis vinifera) by isotope-dilution liquid chromatography–mass spectrometry

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Abstract

A specific, sensitive, precise, and accurate method for the determination of abscisic acid (ABA) in grapevine leaf tissues is described. The method employs high-performance liquid chromatography and electrospray ionization–mass spectrometry (LC-ESI-MS) in selected ion monitoring mode (SIM) to analyze ABA using a stable isotope-labeled ABA as an internal standard. Absolute recoveries ranged from 72% to 79% using methanol/water pH 5.5 (50:50 v/v) as an extraction solvent. The best efficiency was obtained when the chromatographic separation was carried out by using a porous graphitic carbon (PGC) column. The statistical evaluation of the method was satisfactory in the work range. A relative standard deviation (RDS) of < 5.5% and < 6.0% was obtained for intra-batch and inter-batch comparisons, respectively. As for accuracy, the relative error (%Er) was between −2.7 and 4.3%, and the relative recovery ranged from 95% to 107%.

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References

  1. Bray EA (2002) Plant Cell Environ 25:153–161

    Article  CAS  Google Scholar 

  2. Cutler AJ, Krochko JE (1999) Trends Plant Sci 4:472–478

    Article  Google Scholar 

  3. Xiong LM, Zhu JK (2003) Plant Physiol 133:29–36

    Article  CAS  Google Scholar 

  4. Soar CJ, Speirs J, Maffei SM, Loveys BR (2004) Funct Plant Biol 31:659–669

    Article  CAS  Google Scholar 

  5. Koussa T, Colin L, Broquedis M (2004) J Int Sci Vigne Vin 38:141–146

    CAS  Google Scholar 

  6. McCarthy MG (1997) Aust J Grape Wine Res 3:102–108

    Google Scholar 

  7. Stoll M, Loveys B, Dry P (2000) J Exp Bot 51:1627–1634

    Article  CAS  Google Scholar 

  8. Kamboj JS, Browning G, Blake PS, Quinlan JD, Baker DA (1999) Plant Growth Regul 28:21–27

    Article  CAS  Google Scholar 

  9. Muller A, Duchting P, Weiler EW (2002) Planta 216:44–56

    Article  CAS  Google Scholar 

  10. Birkemeyer C, Kolasa A, Kopka J (2003) J Chromatogr A 993:89–102

    Article  CAS  Google Scholar 

  11. Gomez-Cadenas A, Tadeo FR, Talon M, Primo-Millo E (1996) Plant Physiol 112:401–408

    CAS  Google Scholar 

  12. Gomez-Cadenas A, Pozo OJ, Garcia-Augustin P, Sancho JV (2002) Phytochem Analysis 13:228–234

    Article  CAS  Google Scholar 

  13. Ross ARS, Ambrose SJ, Cutler AJ, Feurtado JA, Kermode AR, Nelson K, Zhou R, Abrams SR (2004) Anal Biochem 329:324–333

    Article  CAS  Google Scholar 

  14. Lopez-Carbonell M, Jauregui O (2005) Plant Physiol Bioch 43:407–411

    CAS  Google Scholar 

  15. Zhou R, Squires TM, Ambrose SJ, Abrams SR, Ross ARS, Cutler AJ (2003) J Chromatogr A 1010:75–85

    Article  CAS  Google Scholar 

  16. Jemal M, Schuster A, Whigan DB (2003) Rapid Commun Mass Spectrom 17:1723–1734

    Article  CAS  Google Scholar 

  17. Liu RH, Lin DL, Chang WT, Liu CR, Tsay WI, Li JH, Kuo TL (2002) Anal Chem 74:618A–626A

    CAS  Google Scholar 

  18. Abrams SR, Nelson K, Ambrose SJ (2003) J Label Compd Radiopharm 46:273–283

    Article  CAS  Google Scholar 

  19. Food and Drug Administration (1995) International Conference on Harmonisation [ICH]. Validation of analytical procedures: definitions and terminology, Q2A. Federal Register 60:11260

    Google Scholar 

  20. Food and Drug Administration (1997) International Conference on Harmonisation [ICH]. Validation of analytical procedures: methodology, Q2B. Federal Register 62:27464

    Google Scholar 

  21. Baker SE, Olsson AO, Needham LL, Barr DB (2005) Anal Bioanal Chem 383:963–976

    Article  CAS  Google Scholar 

  22. Garcia I, Ortiz MC, Sarabia L, Vilches C, Gredilla E (2003) J Chromatogr A 992:11–27

    Article  CAS  Google Scholar 

  23. Feinberg M, Boulanger B, Dewe W, Hubert P (2004) Anal Bioanal Chem 380:502–514

    Article  CAS  Google Scholar 

  24. Mauch-Mani B, Mauch F (2005) Curr Opin Plant Biol 8:409–414

    Article  CAS  Google Scholar 

Download references

Acknowledgement

The authors are grateful to the Departament de Tecnologia Frutícola of the Institut de Recerca i Tecnología Agroalimentàries (IRTA) for providing grapevine leaf samples.

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Authors and Affiliations

  1. Centre UdL-IRTA, Rovira Roure 191, 25198, Lleida, Spain

    Francisca Vilaró

  2. Chemistry Department, Lleida University, Rovira Roure 191, 25198, Lleida, Spain

    Anna Canela-Xandri & Ramon Canela

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  1. Francisca Vilaró
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  2. Anna Canela-Xandri
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  3. Ramon Canela
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Correspondence to Ramon Canela.

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Vilaró, F., Canela-Xandri, A. & Canela, R. Quantification of abscisic acid in grapevine leaf (Vitis vinifera) by isotope-dilution liquid chromatography–mass spectrometry. Anal Bioanal Chem 386, 306–312 (2006). https://doi.org/10.1007/s00216-006-0664-2

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  • DOI: https://doi.org/10.1007/s00216-006-0664-2

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