Abstract
Short oxidized multi-walled carbon nanotubes (CNT) were derivatized with fluorescein isothiocyanate (FITC). Capillary electrophoresis coupled with laser-induced fluorescence (CE–LIF) was then used to separate and detect the fluorescently labeled carbon-nanotube probes (CNTP) in multidrug-resistant cells (K562A) and the parent cells (K562S). Greater expression of P-glycoprotein in K562A cells than in K562S cells was confirmed by use of anti-P-glycoprotein antibody and flow-cytometric analysis. Analyses of CNTP in both cell lines using both CE–LIF and flow cytometry showed that CNTP could traverse the cellular membrane without being pumped out by P-glycoprotein. The CNTP distributed in both cell lines was analyzed at the single cell level and the results were compared with those from analysis of ten cells and of the lysate from bulk cells. The results revealed the CE–LIF method could be used for quantitative analysis of CNT in single cells in studies of drug delivery and multidrug resistance.
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References
Kam N, Liu ZA, Dai HJ (2006) Angew Chem Int Edit 45:577–581
Kam N, O’Connell M, Wisdom JA, Dai HJ (2005) Proc Natl Acad Sci USA 102:11600–11605
Kam N, Jessop TC, Wender PA, Dai HJ (2004) J Am Chem Soc 126:6850–6851
LaVan DA, McGuire T, Langer R (2003) Nat Biotechnol 21:1184–1191
Martin CR, Kohli P (2003) Nat Rev Drug Discov 2:29–37
Son SJ, Reichel J, He B, Schuchman M, Lee SB (2005) J Am Chem Soc 127:7316–7317
Bianco A, Prato M (2003) Adv Mater 15:1765–1768
Pastorin G, Wu W, Wieckowski S, Briand JP, Kostarelos K, Prato M, Bianco A (2006) Chem Commun 1182–1184
Bianco A, Kostarelos K, Partidos CD, Prato M (2005) Chem Commun 571–577
Pantarotto D, Singh R, McCarthy D, Erhardt M, Briand JP, Prato M, Kostarelos K, Bianco A (2004) Angew Chem Int Ed 43:5242–5246
Bianco A, Hoebeke J, Godefroy S, Chaloin O, Pantarotto D, Briand JP, Muller S, Prato M, Partidos CD (2005) J Am Chem Soc 127:58–59
Wu W, Wieckowski S, Pastorin G, Benincasa M, Klumpp C, Briand JP, Gennaro R, Prato M, Bianco A (2005) Angew Chem Int Ed 44:6358–6362
Kam N, Dai HJ (2005) J Am Chem Soc 127:6021–6026
Panhuis M (2003) Chem Biol 10:897–898
Gottesman MM, Fojo T, Bates SE (2002) Nat Rev Cancer 2:48–58
Persidis A (1999) Nat Biotechnol 17:94–95
Simon SM, Schindler M (1994) Proc Natl Acad Sci USA 91:3497–3504
Hamilton KO, Backstrom G, Yazdanian MA, Audus KL (2001) J Pharm Sci 90:647–658
Swerts K, De Moerloose B, Dhooge C, Laureys G, Benoit Y, Philippe J (2006) Eur J Cancer 42:295–309
Xiao H, Li X, Zou HF, Yang L, Yang YQ, Wang YL, Wang HL, Le XC (2006) Anal Chim Acta 556:340–346
Xiao H, Li X, Zou HF, Yang L, Wang YL, Wang HL, Le XC (2006) Electrophoresis, in press
Hjertén S (1985) J Chromatogr 347:191–198
Wang Y, Wu J, Wei F (2003) Carbon 41:2939–2948
Pan CS, Xu SY, Hu LG, Su XY, Ou JJ, Zou HF, Guo Z, Zhang Y, Guo BC (2005) J Am Soc Mass Spectrom 16:883–892
Doorn SK, Fields RE, Hu H, Hamon MA, Haddon RC, Selegue JP, Majidi V (2002) J Am Chem Soc 124:3169–3174
Doorn SK, Strano MS, O’Connell MJ, Haroz EH, Rialon KL, Hauge RH, Smalley RE (2003) J Phys Chem B 107:6063–6069
Stuart JN, Sweedler JV (2003) LC–GC Eur 16:427–429
Stuart JN, Sweedler JV (2003) Anal Bioanal Chem 375:28–29
Acknowledgements
Financial support from the National Natural Sciences Foundation of China (No. 20520120220), the China State Key Basic Research Program Grants (2003CB716002), and the Knowledge Innovation Program of DICP to H.Z. are gratefully acknowledged.
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Xiao, H., Yang, L., Zou, H. et al. Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence. Anal Bioanal Chem 387, 119–126 (2007). https://doi.org/10.1007/s00216-006-0654-4
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DOI: https://doi.org/10.1007/s00216-006-0654-4