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Elucidation of n-butyl benzyl phthalate biodegradation using high-performance liquid chromatography and gas chromatography–mass spectrometry

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Abstract

n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. A bacterium species capable of using BBP as the sole source of carbon and energy was isolated from mangrove sediment. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied. The optimum pH, temperature, and salinity for the BBP biodegradation were 7.0, 37°C, and 15 g L−1, respectively. BBP was completely degraded within 6 days under optimum conditions, and the biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP biodegradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid, and benzoic acid by using high-performance liquid chromatography and gas chromatography–mass spectrometry. A preliminary metabolic pathway was proposed for the biodegradation of BBP.

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Authors and Affiliations

  1. Department of Ecology & Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong, People’s Republic of China

    Xiang-Rong Xu, Hua-Bin Li & Ji-Dong Gu

  2. The Swire Institute of Marine Science, The University of Hong Kong, Shek O, Cape d’Aguilar, Hong Kong, China

    Ji-Dong Gu

Authors
  1. Xiang-Rong Xu
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  2. Hua-Bin Li
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  3. Ji-Dong Gu
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Correspondence to Xiang-Rong Xu.

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Xu, XR., Li, HB. & Gu, JD. Elucidation of n-butyl benzyl phthalate biodegradation using high-performance liquid chromatography and gas chromatography–mass spectrometry. Anal Bioanal Chem 386, 370–375 (2006). https://doi.org/10.1007/s00216-006-0627-7

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  • DOI: https://doi.org/10.1007/s00216-006-0627-7

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