Abstract
A new fluorescence method for the detection of proteins with novel composite nanoparticles (CdS/PPA) has been developed. The composite nanoparticles have been prepared through an in-situ polymerization method under ultrasonic irradiation. The surface of the composite nanoparticles was covered with functional groups (-COOH). These groups may play a major role in the improving the water solubility and biocompatibility of the nanoparticles. The composite particles is combined with proteins in NaAc-HCl buffer solution (pH=1.99), which can result in strong fluorescence, and the response is linearly proportional to the concentration of proteins. In λem/λex=650 nm/365 nm place (the stoke’ shift is 285 nm), its fluorescent strength reaches the maximum. Under the optimum conditions, the linear range is 0.10–20.0 μg·ml−1 with the detection limit of 41 ng·ml−1 for HSA, and 0.10–15.0 μg·ml−1 with the detection limit of 35 ng·ml−1 for Human γ-IgG . The method has been applied to the determination of the total protein in human serum samples collected from the hospital and the results are satisfactory.
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Acknowledgements
This work was supported by Natural Science Foundation of China (2075001, 20575001), the Education Department of Anhui Province (No.2006KJ006TD) and the Science Foundation of the Education Department of Anhui Province for Young Teachers (2006jql050zd).
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Chen, HQ., Wang, L., Liu, Y. et al. Preparation of a novel composite particles and its application in the fluorescent detection of proteins. Anal Bioanal Chem 385, 1457–1461 (2006). https://doi.org/10.1007/s00216-006-0610-3
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DOI: https://doi.org/10.1007/s00216-006-0610-3