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UPLC-MS/MS determination of doxazosine in human plasma

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Abstract

A sensitive, selective and rapid method for the analysis of doxazosine (DOX) in human plasma based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is described. DOX and tamsulosin, used as an internal standard (IS), were extracted by liquid-liquid extraction, and the chromatography was performed on a C18 UPLC column packed with 1.7 μm particles. The total run time was 2 min. Detection was achieved by the multiple reaction monitoring of the following transitions: m/z 452→344 and m/z 409→228 for DOX and IS, respectively. Transitions of m/z 452→247 and m/z 409→271 were also collected for confirmation purposes. The calibration curve based on peak area ratio was linear up to at least 100 ng ml−1, with a detection limit of 0.02 ng ml−1 (a signal-to-noise ratio of 3). The method showed satisfactory reproducibility, and the short-term stability of the analyte was assessed. The method was successfully applied to the analysis of DOX in human plasma.

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Authors and Affiliations

  1. National Laboratory for Newborn Screening, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354 (MBC-03), Riyadh, 11211, Saudi Arabia

    Osama Y. Al-Dirbashi, Minnie Jacob, Khalid Al-Qahtani & Mohamed S. Rashed

  2. Pharmaceutical and Medicinal Chemistry Department, National Research Center, Cairo, 12311, Egypt

    Hassan Y. Aboul-Enein

Authors
  1. Osama Y. Al-Dirbashi
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  2. Hassan Y. Aboul-Enein
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  3. Minnie Jacob
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  4. Khalid Al-Qahtani
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  5. Mohamed S. Rashed
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Correspondence to Mohamed S. Rashed.

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Al-Dirbashi, O.Y., Aboul-Enein, H.Y., Jacob, M. et al. UPLC-MS/MS determination of doxazosine in human plasma. Anal Bioanal Chem 385, 1439–1443 (2006). https://doi.org/10.1007/s00216-006-0553-8

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  • DOI: https://doi.org/10.1007/s00216-006-0553-8

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