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Separation and determination of honokiol and magnolol in herbal medicines by flow injection-capillary electrophoresis

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Abstract

A simple, rapid, and accurate method for the separation and determination of honokiol and magnolol in Magnolia officinalis and related herbal medicines was developed by combination of flow injection (FI) and capillary zone electrophoresis (CZE). The analysis was carried out using an unmodified fused-silica capillary (50-μm I.D.; total length 7.5 cm; effective length 4.5 cm). A series of optimization steps afforded the following conditions: the sample solvent consisted of 150 mM NaOH and a running buffer composed of 10 mM sodium tetraborate/10 mM sodium dihydrogenphosphate (NaH2PO4) at pH 12 was applied for the separation of the analytes. The separation could be achieved within 5 min with a sample throughput rate of up to 28 h−1. The repeatability (defined as the relative standard deviation, RSD) for honokiol and magnolol was 2.0% and 1.6% with peak area evaluation, 3.6% and 2.0% with peak height evaluation, and 2.0% and 1.4% with migration time evaluation, respectively. Regression equations revealed linear relationships (r = 0.9991–0.9998) between the peak area of each analyte and the concentration.

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Acknowledgement

We kindly acknowledge the National Science Foundation of China (No. 20275014) for supporting this work.

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Authors and Affiliations

  1. Department of Chemistry, Lanzhou University, Lanzhou, 730000, China

    Lihong Liu, Liuyin Fan, Xingguo Chen & Zhide Hu

  2. Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou, 730000, China

    Xinan Wu

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  1. Lihong Liu
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  2. Xinan Wu
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  3. Liuyin Fan
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  4. Xingguo Chen
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  5. Zhide Hu
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Correspondence to Xingguo Chen.

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Liu, L., Wu, X., Fan, L. et al. Separation and determination of honokiol and magnolol in herbal medicines by flow injection-capillary electrophoresis. Anal Bioanal Chem 384, 1533–1539 (2006). https://doi.org/10.1007/s00216-006-0319-3

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  • DOI: https://doi.org/10.1007/s00216-006-0319-3

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