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Fluorescence ratiometric pH sensor prepared from covalently immobilized porphyrin and benzothioxanthene

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Abstract

A fluorescence ratiometric sensor for pH determination is described in this paper. The sensor incorporated the pH-sensitive dye meso-5,10,15,20-tetra-(4-allyloxyphenyl)porphyrin (TAPP) as an indicator and a pH-insensitive dye N-(2-methacryloxyethyl)benzo[k,l]thioxanthene-3,4-dicarboximide (MBTD), a benzothioxanthene derivative, as a reference for fluorescence ratiometric measurement. To prevent leakage of the dyes, both were photocopolymerized with acrylamide, hydroxyethyl methacrylate, and triethylene glycol dimethacrylate on the silanized glass surface. The reproducibility and response time of the prepared sensor were sufficient. Most common coexisting inorganic ions and organic compounds did not interfere with pH sensing. In the acidic pH range from 1.5 to 5.0 the fluorescence intensity ratio of the two dyes varied linearly as a function of pH. The sensing membrane was found to have a lifetime of at least one month. The sensor was applied to the analysis of waste water and artificial samples.

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References

  1. Ronnie MA, Kjell C, Anders L, Hjalmar B (2000) Anal Biochem 283:104–110

    Article  PubMed  Google Scholar 

  2. Jeevarajan AS, Vani S, Taylor TD, Anderson MM (2002) Biotechnol Bioeng 78:467–472

    Article  PubMed  CAS  Google Scholar 

  3. Grant SA, Glass RS (1997) Sens Actuators B 45:35–42

    Article  Google Scholar 

  4. Iga M, Seki A, Kubota Y, Watanabe K (2003) Sens Actuators B 96:234–238

    Article  CAS  Google Scholar 

  5. Ma LY, Wang HY, Xie H, Xu LX (2004) Spectrochim Acta A 60:1865–1872

    Article  CAS  Google Scholar 

  6. Cui DW, Qian XH, Liu FY, Zhang R (2004) Org Lett 6:2757–2760

    Article  PubMed  CAS  Google Scholar 

  7. Song A, Parus S, Kopelman R (1997) Anal Chem 69:863–867

    Article  PubMed  CAS  Google Scholar 

  8. Xu Z, Rollins A, Alcala R, Marchant RE (1998) J Biomed Mater Res 39:9–15

    Article  PubMed  CAS  Google Scholar 

  9. Kermis HR, Kostov Y, Harms P, Rao G (2002) Biotechnol Prog 18:1047–1053

    Article  PubMed  CAS  Google Scholar 

  10. Oh YK, Straubinger RM (1996) Infect Immun 64:319–325

    PubMed  CAS  Google Scholar 

  11. McNamara KP, Nguyen T, Dumitrascu G, Ji J, Rosenzweig N, Rosenzweig Z (2001) Anal Chem 73:3240–3246

    Article  PubMed  CAS  Google Scholar 

  12. Clark HA, Tjalkens R, Kopelman R, Philbert MA (1999) Anal Chem 71:4837–4843

    Article  PubMed  CAS  Google Scholar 

  13. Ji J, Rosenzweig N, Griffin C, Rosenzweig Z (2000) Anal Chem 72:3497–3503

    Article  PubMed  CAS  Google Scholar 

  14. Potyrailo RA, Hieftje GM (1998) Anal Chim Acta 370:1–8

    Article  CAS  Google Scholar 

  15. Amao Y, Miyashita T, Okura I (2000) Analyst 125:871–875

    Article  CAS  Google Scholar 

  16. Zhang XB, Guo CC, Li ZZ, Shen GL, Yu RQ (2002) Anal Chem 74:821–825

    Article  PubMed  CAS  Google Scholar 

  17. Yang RH, Wang KM, Long LP, Xiao D, Yang XH, Tan WH (2002) Anal Chem 74:1088–1096

    Article  PubMed  CAS  Google Scholar 

  18. Zhang XB, Li ZZ, Guo CC, Chen SH, Shen GL, Yu RQ (2001) Anal Chim Acta 439:65–71

    Article  CAS  Google Scholar 

  19. Yang RH, Wang KM, Xiao D, Yang XH (2000) Analyst 125:1441–1445

    Article  CAS  Google Scholar 

  20. Papkovsky DB, Ponomarev GV, Wolfbeis OS (1997) J Photochem Photobiol 104:151–158

    Article  CAS  Google Scholar 

  21. Igarash S, Kuwae K, Yotsuyanagi T (1994) Anal Sci 10:821—822

    Article  Google Scholar 

  22. Blair TL, Allen JR, Daunert S, Bachas LG (1993) Anal Chem 65:2155–2158

    Article  CAS  Google Scholar 

  23. Mao P, Qian XH, Zhang HZ, Yao W (2004) Dyes Pigment 60:9–16

    Article  CAS  Google Scholar 

  24. Qian XH, Mao P, Yao W, Guo XF (2002) Tetrahedron Lett 43:2995–2998

    Article  CAS  Google Scholar 

  25. Xavier MP, Garcia-Fresnadillo D, Moreno-Bondi MC, Orellana G (1998) Anal Chem 70:5184–5189

    Article  CAS  Google Scholar 

  26. Niu CG, Zeng GM, Chen LX, Shen LX, Yu RQ (2004) Analyst 129:20–24

    Article  PubMed  CAS  Google Scholar 

  27. Lobnik A, Oehme I, Murkovic I, Wolfbeis OS (1998) Anal Chim Acta 367:159–165

    Article  CAS  Google Scholar 

  28. Bernhard DD, Mall S, Pantano P (2001) Anal Chem 73:2484–2490

    Article  PubMed  CAS  Google Scholar 

  29. Niu CG, Guan AL, Zeng GM, Liu YG, Huang GH, Gao PF, Gui XQ (2005) Anal Chim Acta (in press)

  30. Dean JA (eds) (1985) Lange’s handbook of chemistry, 13th edn. McGraw–Hill, New York, Sec. 5.102, Tables 5–24

  31. Yan XB, Weng M, Zhang MH, Shen T (1997) Sci China (Chinese) 27:282–288

    Google Scholar 

  32. Niu CG, Li ZZ, Zhang XB, Lin WQ, Shen GL, Yu RQ (2002) Anal Bioanal Chem 372:519–524

    Article  PubMed  CAS  Google Scholar 

  33. Munkholm C, Walt DR, Milanovich FP, Klainer SM (1986) Anal Chem 58:1427–1430

    Article  CAS  Google Scholar 

  34. Manna BK, Bera SC, Rohatgi-Mukherjee KK (1995) Spectrochim Acta Part A 51:1051–1060

    Article  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (70171055, 50179011), the Natural Foundation for Distinguished Young Scholars (Grant No. 50225926), the Doctoral Foundation of Ministry of Education of China (20020532017), the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE, P.R.C. (TRAPOYT) in 2000, the National 863 High Technology Research Program of China (2001AA644020, 2003AA644010), and the Science Foundation of Hunan University (521101618).

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Authors and Affiliations

  1. Department of Environmental Science and Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, P. R. China

    Cheng-Gang Niu, Xiao-Qin Gui, Guang-Ming Zeng, Ai-Ling Guan, Pan-Feng Gao & Pin-Zhu Qin

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  1. Cheng-Gang Niu
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  2. Xiao-Qin Gui
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Correspondence to Cheng-Gang Niu.

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Niu, CG., Gui, XQ., Zeng, GM. et al. Fluorescence ratiometric pH sensor prepared from covalently immobilized porphyrin and benzothioxanthene. Anal Bioanal Chem 383, 349–357 (2005). https://doi.org/10.1007/s00216-005-3422-y

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  • DOI: https://doi.org/10.1007/s00216-005-3422-y

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