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Single-molecule fluorescence detection in microfluidic channels—the Holy Grail in μTAS?

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Abstract

Both single-molecule detection (SMD) methods and miniaturization technologies have developed very rapidly over the last ten years. By merging these two techniques, it may be possible to achieve the optimal requirements for the analysis and manipulation of samples on a single molecule scale. While miniaturized structures and channels provide the interface required to handle small particles and molecules, SMD permits the discovery, localization, counting and identification of compounds. Widespread applications, across various bioscience/analytical science fields, such as DNA-analysis, cytometry and drug screening, are envisaged. In this review, the unique benefits of single fluorescent molecule detection in microfluidic channels are presented. Recent and possible future applications are discussed.

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Abbreviations

FACS :

Fluorescence activated cell sorter

FCS :

Fluorescence correlation spectroscopy

(sp) FRET:

(Single pair) fluorescence resonance energy transfer

GFP :

Green fluorescent protein

μTAS :

Micro total analysis system

SMD :

Single-molecule detection

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Acknowledgements

Financial support by the European Community (CellPROM project, contract No. NMP4-CT-2004-500039 under the 6th Framework Programme for Research and Technological Development) is gratefully acknowledged. We thank Kaoru Tachikawa for help with the manuscript. The experimental data presented here were acquired partly at the Max-Planck Institute of Biophysical Chemistry, Göttingen (Germany) and by the Experimental Biophysics Group (Petra Schwille).

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  1. Department of Miniaturization, Institute for Analytical Sciences (ISAS), Bunsen-Kirchhoff-Str. 11, 44139, Dortmund, Germany

    Petra S. Dittrich & Andreas Manz

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  1. Petra S. Dittrich
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  2. Andreas Manz
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Correspondence to Petra S. Dittrich.

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Dedicated to the memory of Wilhelm Fresenius

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Dittrich, P.S., Manz, A. Single-molecule fluorescence detection in microfluidic channels—the Holy Grail in μTAS?. Anal Bioanal Chem 382, 1771–1782 (2005). https://doi.org/10.1007/s00216-005-3335-9

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  • DOI: https://doi.org/10.1007/s00216-005-3335-9

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