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Fiber-optic nanosensors for single-cell monitoring

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Abstract

This article is an overview of the fabrication, operating principles, and applications of fiber-optic nanobiosensors with the capability of in-vivo analysis at the single-cell level. Recently, the cross-disciplinary integration of nanotechnology, biology, and photonics has been revolutionizing important areas in molecular biology, especially diagnostics and therapy at the molecular and cellular level. Fiber-optic nanobiosensors are a unique class of biosensor that enable analytical measurements in individual living cells and the probing of individual chemical species in specific locations within a cell. This article provides a review of the research performed in our laboratory and discusses the usefulness and potential of this nanotechnology-based biosensor system in biological research and its applications to biomonitoring of individual cells.

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References

  1. Vo-Dinh T, Sepaniak MJ, Griffin GD, Alarie JP (1993) Immunosensors: principles and applications, in immuno methods. Academic Press, NY, pp 85–92

    Google Scholar 

  2. Vo-Dinh T, Griffin GD, Sepaniak MJ (1991) In: Wolfbeis OS (ed) Fiber optic immunosensors, in CRC handbook: chemical sensors and biosensors. CRC Press, Boca Raton, FL, USA

  3. Vo-Dinh T et al (1987) Antibody-based fiber optics biosensor for the carcinogen benzo[a]pyrene. Appl Spectrosc 41(5):735–738

    CAS  Google Scholar 

  4. Betzig E, Trautman JK, Harris TD, Weiner JS, Kostelak RL (1991) Breaking the diffraction barrier—optical microscopy on a nanometric scale. Science 251(5000):1468–1470

    Google Scholar 

  5. Betzig E, Chichester RJ (1993) Single molecules observed by near-field scanning optical microscopy. Science 262(5138):1422–1425

    CAS  Google Scholar 

  6. Tan W, Shi ZY, Smith S, Birnbaum D, Kopelman R (1992) Submicrometer intracellular chemical optical fiber sensors. Science 258(5083):778–781

    CAS  PubMed  Google Scholar 

  7. Tan W, Shi ZY, Smith S, Kopelman R (1992) Development of submicron chemical optic sensors. Anal Chem 64(23):2985–2990

    CAS  Google Scholar 

  8. Zeisel D, Deckert V, Zenobi R, Vo-Dinh T (1998) Near-field surface-enhanced Raman spectroscopy of dye molecules adsorbed on silver island films. Chem Phys Lett 283(5–6):381–385

    CAS  Google Scholar 

  9. Deckert V, Zeisel D, Zenobi R, Vo-Dinh T (1998) Near-field surface enhanced Raman imaging of dye-labeled DNA with 100-nm resolution. Anal Chem 70(13):2646–2650

    CAS  Google Scholar 

  10. Cullum B, Griffin GD, Miller GH, Vo-Dinh T (2000) Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors. Anal Biochem 277(1):25–32

    CAS  PubMed  Google Scholar 

  11. Kasili PM et al (2002) Nanosensor for in-vivo measurement of the carcinogen benzo[a]pyrene in a single cell. J Nanosci Nanotechnol 2(6):653–658

    CAS  PubMed  Google Scholar 

  12. Vo-Dinh T, Alarie JP, Cullum BM, Griffin GD (2000) Antibody-based nanoprobe for measurement of a fluorescent analyze in a single cell. Nat Biotechnol 18(7):764–767

    CAS  PubMed  Google Scholar 

  13. Vo-Dinh T, Griffin GD, Alarie JP, Cullum BM, Sumpter B, Noid DJ (2000) Development of nanosensors and bioprobes. J Nanoparticle Res 2:17–27

    CAS  Google Scholar 

  14. Vo-Dinh T, Cullum B (2000) Biosensors and biochips: advances in biological and medical diagnostics. Fresenius J Anal Chem 366(6–7):540–551

    CAS  PubMed  Google Scholar 

  15. Nice EC, Catimel B (1999) Instrumental biosensors: new perspectives for the analysis of biomolecular interactions. Bioessays 21(4):339–352

    CAS  PubMed  Google Scholar 

  16. Weetall HH (1999) Chemical sensors and biosensors, update, what, where, when and how. Biosens Bioelectron 14(2):237–242

    CAS  Google Scholar 

  17. Tess ME, Cox JA (1999) Chemical and biochemical sensors based on advances in materials chemistry. J Pharm Biomed Anal 19(1–2):55–68

    CAS  PubMed  Google Scholar 

  18. Braguglia CM (1998) Biosensors: an outline of general principles and application. Chem Biochem Eng Q 12(4):183–190

    CAS  Google Scholar 

  19. Cullum B, Vo-Dinh T (2000) The development of optical nanosensors for biological measurements. Trends Biotechnol 18(9):388–393

    CAS  PubMed  Google Scholar 

  20. Kasili PM, Song JM, Vo-Dinh T (2004) Optical sensor for the detection of caspase-9 activity in a single cell. J Am Chem Soc 9(126):2799–806

    Article  Google Scholar 

  21. Song JM et al (2004) Detection of cytochrome c in a single cell using an optical nanobiosensor. Anal Chem 76(9):2591–2594

    CAS  PubMed  Google Scholar 

  22. Vo-Dinh T (1989) Chemical analysis of polycyclic aromatic compounds. Wiley, NY

    Google Scholar 

  23. Alarie J, Vo-Dinh T (1996) Antibody-based submicron biosensor for benzo[a]pyrene DNA adduct. Polycyclic Aromatic Compounds 8(1):45–52

    CAS  Google Scholar 

  24. Alarie JP, Sepaniak MJ, Vo-Dinh T (1990) Evaluation of antibody immobilization techniques for fiber optic-based fluoroimmunosensing. Anal Chim Acta 229(2):169–176

    CAS  Google Scholar 

  25. Vo-Dinh T, Tromberg BJ, Griffin GD, Ambrose KR, Sepaniak MJ, Gardenhire EM (1987) Antibody fiber optics biosensor for the carcinogen benzo(a)pyrene. Appl Spectroscopy 5(41):735

    Google Scholar 

  26. Nicholson D, Thornberry NA (1997) Caspases: killer proteases. Trends Biochem Sci 8:299–306

    Article  Google Scholar 

  27. Cohen GMAP (1997)—Caspases: the executioners of apoptosis. Biochem J 326(Pt 1):1–16

    CAS  PubMed  Google Scholar 

  28. Kasili PM, Song JM, Vo-Dinh T (2004) Optical sensor for the detection of caspase-9 activity in a single cell. J Am Chem Soc 9(126):2799–806

    Article  Google Scholar 

  29. Noodt B, Berg K, Stokke T (1996) Apoptosis and necrosis induced with light and 5-aminolaevulinic acid-derived protoporphyrin IX. Br J Cancer 74(1):22–29

    CAS  PubMed  Google Scholar 

  30. Tan WH, Shi ZY, Kopelman R (1992) Development of submicron chemical fiber-optic sensors. Anal Chem 64(23):2985–2990

    CAS  Google Scholar 

  31. Tan WH et al (1992) Submicrometer intracellular chemical optical fiber sensors. Science 258(5083):778–781

    CAS  PubMed  Google Scholar 

  32. Samuel J et al (1994) Miniaturization of organically doped sol-gel materials—a microns-size fluorescent ph sensor. Materials Lett 21(5–6):431–434

    CAS  Google Scholar 

  33. McCulloch SaUD (1995) IEE Proceedings-Optoelectronics, vol 144. p 162

  34. Tan WH, Shi ZY, Kopelman R (1995) Miniaturized fiber-optic chemical sensors with fluorescent dye—doped polymers. Sens Actuators B-Chem 28(2):157–163

    Article  Google Scholar 

  35. Song A, Parus S, Kopelman R (1997) High-performance fiber optic pH microsensors for practical physiological measurements using a dual-emission sensitive dye. Anal Chem 69(5):863–867

    CAS  PubMed  Google Scholar 

  36. Koronczi I et al (1998) Development of a submicron optochemical potassium sensor with enhanced stability due to internal reference. Sens Actuators B-Chem 51(1–3):188–195

    Article  Google Scholar 

  37. Bui JD et al (1999) Probing intracellular dynamics in living cells with near-field optics. J Neurosci Methods 89(1):9–15

    CAS  PubMed  Google Scholar 

  38. Barker SLR, Kopelman R (1998) Development and cellular applications of fiber optic nitric oxide sensors based on a gold-adsorbed fluorophore. Anal Chem 70(23):4902–4906

    CAS  PubMed  Google Scholar 

  39. Munkholm C, Walt DR, Milanovich FP (1987) Preparation of co2 fiber-optic chemical sensor. Abstr Papers Am Chem Soc 193:183-ANYL

    Google Scholar 

  40. Munkholm C, Parkinson DR, Walt DR (1990) Intramolecular fluorescence self-quenching of fluoresceinamine. J Am Chem Soc 112(7):2608–2612

    CAS  Google Scholar 

  41. Barker SLR, Thorsrud BA, Kopelman R (1998) Nitrite- and chloride-selective fluorescent nano-optodes and in in vitro application to rat conceptuses. Anal Chem 70(1):100–104

    CAS  PubMed  Google Scholar 

  42. Tan WH et al (1999) Ultrasmall for cellular. Anal Chem 71(17):606A–612A

    CAS  PubMed  Google Scholar 

  43. Barker SLR et al (1998) Fiber-optic nitric oxide-selective biosensors and nanosensors. Anal Chem 70(5):971–976

    CAS  PubMed  Google Scholar 

  44. Vo-Dinh T, Cullum BM (2003) CRC handbook for biomedical photonics. In: Vo-Dinh T (ed) Nanosensors for single-cell analysis, vol 14. CRC Press, NY

  45. Barker S, Kopelman R (1998) Development and cellular applications of fiber optic nitric oxide sensors based on a gold-adsorbed fluorophore. Anal Chem 70(23):4902–4906

    CAS  PubMed  Google Scholar 

  46. Tan W, Shi ZY, Kopelman R (1992) Development of submicron chemical fiber-optic sensors. Anal Chem 64(23):2985–2990

    CAS  Google Scholar 

  47. Uttamchandani D, McCulloch S (1996) Optical nanosensors—towards the development of intracellular monitoring. Adv Drug Delivery Rev 21:239–247

    CAS  Google Scholar 

  48. Vo-Dinh T (2002) Nanobiosensors: probing the sanctuary of individual living cells. J Cellular Biochem 39(Supp 161):154

    Article  Google Scholar 

  49. Shortreed M, Kopelman R, Kunh M, Hoyland B (1996) Fluorescent fiber-optic calcium sensor for physiological measurements. Anal Chem 68(8):1414–1418

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Oak Ridge National Laboratory, Advanced Biomedical Science and Technology Group, Oak Ridge, TN 37831-6101, USA

    Tuan Vo-Dinh & Paul Kasili

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  1. Tuan Vo-Dinh
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  2. Paul Kasili
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Correspondence to Tuan Vo-Dinh.

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Vo-Dinh, T., Kasili, P. Fiber-optic nanosensors for single-cell monitoring. Anal Bioanal Chem 382, 918–925 (2005). https://doi.org/10.1007/s00216-005-3256-7

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  • DOI: https://doi.org/10.1007/s00216-005-3256-7

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