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Pre-column derivatization of rofecoxib for determination in serum by HPLC

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Abstract

An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid–liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10–500 ng ml−1.

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Acknowledgements

This work was supported by grants from the Research Council of Tehran University of Medical Sciences and the Iran Chapter of TWAS.

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Authors and Affiliations

  1. Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    M. Amini, M. PirAli Hamedani, M. Vosooghi, M. Nabavi & A. Shafiee

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  1. M. Amini
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  2. M. PirAli Hamedani
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  3. M. Vosooghi
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  4. M. Nabavi
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  5. A. Shafiee
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Correspondence to A. Shafiee.

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Amini, M., Hamedani, M.P., Vosooghi, M. et al. Pre-column derivatization of rofecoxib for determination in serum by HPLC. Anal Bioanal Chem 382, 1265–1268 (2005). https://doi.org/10.1007/s00216-005-3249-6

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  • DOI: https://doi.org/10.1007/s00216-005-3249-6

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