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One-step synthesis of amino-dextran-protected gold and silver nanoparticles and its application in biosensors

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Abstract

A sensitive method for the detection of the lectin protein concanavalin A (Con A) was developed using amino-dextran (AD)-protected gold (AD-Au) and silver nanoparticles (AD-Ag) as sensitive optical probes. The AD-Au and AD-Ag nanoparticles were synthesized by directly applying amino-dextran as a reductive and protective reagent. The size of the nanoparticles could be altered by changing the molar ratio of AD to the metal salt. The amino-dextran bound to Con A by forming a 4:1 Au-Con A complex at neutral pH, and the nanoparticles were induced to aggregate by Con A. The absorption intensity of the nanoparticles decreased linearly with as the Con A concentration was increased from 3.85×10−8 to 6.15×10−7 M. The Au-Con A complex was dissociated by the disaccharide isomaltose, which has a higher affinities for Con A than Au; this competitive strategy could also be used to detect similar types of saccharides.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grant No. 20475052) and the National Key Basic Research Development Project “Research on Human Major Disease Proteomics” (Grant No. 2001CB5102).

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  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China

    Ying Ma, Nan Li, Cheng Yang & Xiurong Yang

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  1. Ying Ma
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  2. Nan Li
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  3. Cheng Yang
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  4. Xiurong Yang
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Correspondence to Xiurong Yang.

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Ma, Y., Li, N., Yang, C. et al. One-step synthesis of amino-dextran-protected gold and silver nanoparticles and its application in biosensors. Anal Bioanal Chem 382, 1044–1048 (2005). https://doi.org/10.1007/s00216-005-3222-4

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  • DOI: https://doi.org/10.1007/s00216-005-3222-4

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