Abstract
This work describes an electroanalytical investigation of dopamine using cyclic voltammetry (CV) and the graphite–polyurethane composite electrode (GPU). In CV studies, well-defined redox peaks characterize the oxidation process at the GPU electrode, which is indicative of electrocatalytic effects associated with active sites on the GPU electrode surface. A new analytical methodology was developed using the GPU electrode and square wave voltammetry (SWV) in BR buffer solution (0.1 mol L−1; pH 7.4). Analytical curves were constructed under optimized conditions (f=60s−1, ΔEa=50 mV, ΔEI=2 mV) and detection and quantification limits of 6.4×10−8 mol L−1 (12.1 μg L−1) and 5.2×10−6 mol L−1 (0.9 mg L−1), respectively, were achieved. The precision of the method was checked by performing ten successive measurements for a 9.9×10−6 mol L−1 dopamine solution. For intra-assay and inter-assay precisions, the relative standard deviations were 1.9 and 2.3%, respectively. In order to evaluate the developed methodology, the determination of dopamine was performed with good sensitivity and selectivity, without the interference of ascorbic acid in synthetic cerebrospinal fluid, which indicates that the new methodology enables reliable analysis of dopamine.
Similar content being viewed by others
References
Sun Y, Baoxian Y, Zhang W, Zhou X (1998) Anal Chim Acta 363:75–80
Stamford JA, Palij P, Davidson C, Trout SJ (1995) Bioelectrochem Bioenerg 38:289–296
Davidson C, Ellinwood EH, Douglas SB, Lee TH (2000) J Neurol Methods 101:75–83
Ghita M, Arrigan DWM (2004) Electrochim Acta 49:4743–4751
Zhang L, Jia J, Zou X, Dong S (2004) Electroanalysis 16:1413–1418
Zhao H, Zhang Y, Yuan Z (2002) Electroanalysis 14:1031–1034
Raj CR, Tokuda K, Ohsaka T (2001) Bioelectrochemistry 53:183–191
Zhao H, Zhang Y, Yuan Z (2001) Anal Chim Acta 441:117–122
Dávila MM, Elizalde MP, Mattusch J, Wennrich R (2001) Electrochem Acta 46:3189–3197
Liu T, Li M, Li Q (2004) Talanta 63:1053–1059
Roy PR, Okajima T, Ohsaka T (2003) Bioelectrochemistry 59:11–19
Murray RW, Ewing AG, Durst RA (1987) Anal Chem 59:379A–385A
Mendes RK, Claro Neto S, Cavalheiro ETG (2002) Talanta 257:909–917
Oser BL (1966) Hawk’s physiological chemistry. TATA McGraw-Hill, New Delhi
Zhang F, Yang L, Shuping B, Liu J, Liu F, Wang X, Yang X, Gan N, Yu T, Hu J, Li H, Yang T (2001) J Inorg Biol 87:105–113
Bard AJ, Kaulkner LR (1980) Electrochemical methods: fundamentals and applications. Wiley, New York
Gosser DK Jr (1994) Cyclic voltammetry simulation and analysis of reaction mechanisms. VCH Publishers, New York
Lovric M, Komorsky-Lovric S (1988) J Electroanal Chem 248:239–253
Miller JC, Miller JN (1988) Statistics for analytical chemistry, 2nd edn. Ellis Horwood, Chichester
http://www.iupac.org/divisions/V/501/draftoct19.pdf
Xiao L, Chen J, Cha C (2000) J Electroanal Chem 495:27–35
Malinauskas A, Garjonyté R, Maeikiené R, Jureviciuté I (2004) Talanta 64:121–129
Kawagoe TK, Wightman RM (1994) Talanta 41:865–874
Acknowledgements
The authors are indebted to FAPESP (Processes: 01/14629-0, 01/06029-3 and 03/02630-0), CAPES and CNPq.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
de Toledo, R.A., Santos, M.C., Cavalheiro, E.T.G. et al. Determination of dopamine in synthetic cerebrospinal fluid by SWV with a graphite–polyurethane composite electrode. Anal Bioanal Chem 381, 1161–1166 (2005). https://doi.org/10.1007/s00216-005-3066-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-005-3066-y