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Eu3+-doped Gd2O3 nanoparticles as reporters for optical detection and visualization of antibodies patterned by microcontact printing

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Abstract

Lanthanide oxide nanoparticles are promising luminescent probes in bioanalysis, because of their unique spectral properties, photostability, and low-cost synthesis. We report for the first time the application of europium-doped gadolinium oxide (Eu:Gd2O3) nanoparticles to the optical imaging of antibody micropatterns. The nanoparticles were synthesized by spray pyrolysis and coated with antibody (IgG) molecules by physical adsorption. Our experiments showed that the Eu:Gd2O3 is a good biocompatible solid support for antibody immobilization. The antibodies (anti-rabbit IgG) immobilized on the nanoparticles had excellent biological activity in the specific recognition reaction with rabbit IgG patterned in line strips (10 μm×10 μm) on a glass substrate by use of a micro-contact printing technique. The specific immunoreaction was confirmed by two independent microscopic techniques—fluorescence and scanning electron microscopy (SEM). Both microscopic images revealed that the nanoparticles were organized into designated structures as defined by the microcontact printing process with negligible non-specific binding. The nanoparticles can be used as fluorescent markers in a variety of immunosensing applications in a microscale format.

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References

  1. Steinkamp T, Karst U (2004) Anal Bioanal Chem 380:24–30

    Article  CAS  Google Scholar 

  2. Bhargava RN (1996) J Lumin 70:85–94

    Article  CAS  Google Scholar 

  3. Tissue BM (1998) Chem Mater 10:2837–2845

    Article  CAS  Google Scholar 

  4. Diamandis EP, Christopoulos TK (1990) Anal Chem 62:1149A–1157A

    Article  CAS  Google Scholar 

  5. Chan WCW, Maxwell DJ, Gao XH, Bailey RE, Han MY, Nie SM (2002) Curr Opin Biotechnol 13:40–46

    Article  CAS  Google Scholar 

  6. Riegler J, Nann T (2004) Anal Bioanal Chem 379:913–919

    Article  CAS  Google Scholar 

  7. Eilers H, Tissue BM (1996) Chem Phys Lett 251:74–78

    Article  CAS  Google Scholar 

  8. Hao J, Studenikin SA, Cocivera M (2001) J Lumin 93:313–319

    Article  CAS  Google Scholar 

  9. Kang YC, Park SB, Lenggoro IW, Okuyama K (1999) J Phys Chem Solids 60:379–384

    Article  CAS  Google Scholar 

  10. Bazzi R, Flores-Gonzalez MA, Louis C, Lebbou K, Dujardin C, Brenier A, Zhang W, Tillement O, Bernstein E, Perriat P (2003) J Lumin 102–103:445–450

    Article  Google Scholar 

  11. Bian L-j, Xi H-a, Qian X-f, Yin J, Zhu Z-k, Lu Q-h (2002) Mater Res Bull 37:2293–2301

    Article  CAS  Google Scholar 

  12. Kang YC, Roh HS, Park SB, Park HD (2002) J Mater Sci Lett 21:1027–1029

    Article  CAS  Google Scholar 

  13. Dosev D, Guo B, Kennedy IM (2005) Journal of Aerosol Science, in press, available on-line at http://www.sciencedirect.com

  14. Gordon WO, Carter JA, Tissue BM (2004) J Lumin 108:339–342

    Article  CAS  Google Scholar 

  15. Feng J, Shan GM, Maquieira A, Koivunen ME, Guo B, Hammock BD, Kennedy IM (2003) Anal Chem 75:5282–5286

    Article  CAS  Google Scholar 

  16. Beaurepaire E, Buissette V, Sauviat MP, Giaume D, Lahlil K, Mercuri A, Casanova D, Huignard A, Martin JL, Gacoin T, Boilot JP, Alexandrou A (2004) Nano Lett 4:2079–2083

    Article  CAS  Google Scholar 

  17. Espina V, Mehta AI, Winters ME, Calvert V, Wulfkuhle J, Petricoin EF, Liotta LA (2003) Proteomics 3:2091–2100

    Article  CAS  Google Scholar 

  18. Kusnezow W, Hoheisel JD (2002) Biotechniques:14− +

  19. Kane RS, Takayama S, Ostuni E, Ingber DE, Whitesides GM (1999) Biomaterials 20:2363–2376

    Article  CAS  Google Scholar 

  20. Tan JL, Tien J, Chen CS (2002) Langmuir 18:519–523

    Article  CAS  Google Scholar 

  21. Bernard A, Renault JP, Michel B, Bosshard HR, Delamarche E (2000) Adv Mater 12:1067–1070

    Article  CAS  Google Scholar 

  22. Graber DJ, Zieziulewicz TJ, Lawrence DA, Shain W, Turner JN (2003) Langmuir 19:5431–5434

    Article  CAS  Google Scholar 

  23. Lin SC, Tseng FG, Huang HM, Huang CY, Chieng CC (2001) Fresenius J Anal Chem 371:202–208

    Article  CAS  Google Scholar 

  24. Scorilas A, Bjartell A, Lilja H, Moller C, Diamandis EP (2000) Clin Chem 46:1450–1455

    CAS  Google Scholar 

  25. Orth RN, Clark TG, Craighead HG (2003) Biomed Microdevices 5:29–34

    Article  CAS  Google Scholar 

  26. Geho D, Lahar N, Gurnani P, Huebschman M, Herrmann P, Espina V, Shi A, Wulfkuhle J, Garner H, Petricoin E, Liotta LA, Rosenblatt KP (2005) Bioconjug Chem 16:559–566

    Article  CAS  Google Scholar 

  27. Gerion D, Chen FQ, Kannan B, Fu AH, Parak WJ, Chen DJ, Majumdar A, Alivisatos AP (2003) Anal Chem 75:4766–4772

    Article  CAS  Google Scholar 

  28. Dosev D, Nichkova M, Liu M, Liu GY, Hammock BD, Kennedy IM (2005) Journal of Biomedical Optics 10(6):064006-1–064006-7

    Google Scholar 

  29. (http://www.probes.com) Molecular Probes, Amine-reactive Probes

  30. Kumar A, Whitesides GM (1993) Appl Phys Lett 63:2002–2004

    Article  CAS  Google Scholar 

  31. Dosev D, Nichkova M, Liu M, Liu G-y, Hammock BD, Kennedy IM (2005) Proceedings SPIE - The International Society for Optical Engineering, Biomedical Optics, BIOS, San Jose, CA, 2005. 5699:473–481

  32. Shi-Che C, Peron R, Dosev D, Kennedy IM (2004) SPIE Proceedings, BioMEMS and Nanotechnology 5275:186–196

    Google Scholar 

  33. Tapec R, Zhao XJJ, Tan WH (2002) J Nanosci Nanotechnol 2:405–409

    Article  CAS  Google Scholar 

  34. Chan CPY, Bruemmel Y, Seydack M, Sin KK, Wong LW, Merisko-Liversidge E, Trau D, Renneberg, R (2004) Anal Chem 76:3638–3645

    Article  CAS  Google Scholar 

  35. Chan CP-y, Haeussler M, Zhong TB, Dong Y, Sin K-k, Mak W-c, Trau D, Seydack M, Renneberg R (2004) J Immunol Methods 295:111–118

    Article  CAS  Google Scholar 

  36. Mattoussi H, Mauro JM, Goldman ER, Anderson GP, Sundar VC, Mikulec FV, Bawendi MG (2000) J Am Chem Soc 122:12142–12150

    Article  CAS  Google Scholar 

  37. Goldman ER, Anderson GP, Tran PT, Mattoussi H, Charles PT, Mauro JM (2002) Anal Chem 74:841–847

    Article  CAS  Google Scholar 

  38. Goldman ER, Balighian ED, Mattoussi H, Kuno MK, Mauro JM, Tran PT, Anderson GP (2002) J Am Chem Soc 124:6378–6382

    Article  CAS  Google Scholar 

  39. Gao XH, Chan WCW, Nie SM (2002) J Biomed Opt 7:532–537

    Article  CAS  Google Scholar 

  40. Silverton EW, Navia MA, Davies DR (1977) Proceedings of the National Academy of Sciences of the United States of America 74:5140

    Google Scholar 

  41. Amit AG, Mariuzza RA, Phillips SE, Poljak RJ (1986) Science 233:747

    Article  CAS  Google Scholar 

  42. Caruso F, Mohwald H (1999) J Am Chem Soc 121:6039–6046

    Article  CAS  Google Scholar 

  43. Andersson H, Jonsson C, Moberg C, Stemme G (2002) Talanta 56:301–308

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to acknowledge the support of the National Science Foundation, grant DBI-0102662, the Superfund Basic Research Program with Grant 5P42ES04699 from the National Institute of Environmental Health Sciences NIH, and the support of the Cooperative State Research, Education, and Extension Service, US Department of Agriculture, under Award No. 05-35603-16280.

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Authors and Affiliations

  1. Department of Entomology, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA

    Mikaela Nichkova, Shirley J. Gee & Bruce D. Hammock

  2. Department of Mechanical and Aeronautical Engineering, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA

    Dosi Dosev, Richard Perron & Ian M. Kennedy

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  1. Mikaela Nichkova
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  2. Dosi Dosev
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  3. Richard Perron
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  4. Shirley J. Gee
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  5. Bruce D. Hammock
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  6. Ian M. Kennedy
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Correspondence to Mikaela Nichkova.

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Nichkova, M., Dosev, D., Perron, R. et al. Eu3+-doped Gd2O3 nanoparticles as reporters for optical detection and visualization of antibodies patterned by microcontact printing. Anal Bioanal Chem 384, 631–637 (2006). https://doi.org/10.1007/s00216-005-0246-8

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  • DOI: https://doi.org/10.1007/s00216-005-0246-8

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