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Fluorometric determination of thiol redox state

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Abstract

This work presents a fluorometric thiol redox state (TRS) method based on the thiol-specific fluorescent probe monobromobimane. It determines the concentrations of non-protein (NP) thiols glutathione, cysteine and N-acetylcysteine, the protein (P) thiols, as well as the contribution of these components to symmetric and mixed disulfides (NPSSR, NPSSC, NPSSCAc, PSSR, PSSC, PSSCAc, PSSP). The method is very sensitive since it measures as low as 30 pmol -SH groups in samples with a minimum of 1–5 mg total protein, making possible the measurement of oxidative stress–related TRS components even in biological fluids such as cerebrospinal.

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References

  1. Patsoukis N, Zervoudakis G, Georgiou DC, Angelatou F, Matsokis AN, Panagopoulos TN (2004) Epilepsy Res 62:65–74

    Article  PubMed  CAS  Google Scholar 

  2. Alexandris I, F. AS, Vagianos C, Patsoukis N, Georgiou DC, Nikolopoulou V, Scopa CD (2004) Clin Biochem 37:350–356

    Article  PubMed  CAS  Google Scholar 

  3. Patsoukis N, Georgiou DC (2004) Anal Bioanal Chem 378:1783–1792

    Article  PubMed  CAS  Google Scholar 

  4. Ellman LG (1959) Arch Biochem Biophys 82:70–77

    Article  PubMed  CAS  Google Scholar 

  5. Fahey CR, Newton LG (1987) In: Jakoby BW, Griffith WO (eds) Methods in enzymology, vol. 143. Academic, San Diego, pp 85–96

    Google Scholar 

  6. Kosower ME, Kosower SN (1995) In: Packer L (ed) Methods in enzymology, vol. 251. Academic, San Diego, pp 133–148

    Google Scholar 

  7. Bayir H, Kagan EV, Tyurina YY, Tyurin V, Ruppel AR, Adelson PD, Graham HS, Janesko K, Clark SBR, Kochanek MP (2002) Pediatr Res 51:571–578

    Article  PubMed  Google Scholar 

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Acknowledgements

We are grateful to D. Konstantinou from Medical School of the University of Patras, Greece, for providing CSF samples. This work was financially supported by the Greek Ministry of Education, University of Patras, Greece.

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Authors and Affiliations

  1. Section of Genetics, Cell Biology and Development, Department of Biology, University of Patras, 26100, Patras, Greece

    Nikolaos Patsoukis & Christos D. Georgiou

Authors
  1. Nikolaos Patsoukis
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  2. Christos D. Georgiou
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Correspondence to Christos D. Georgiou.

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Patsoukis, N., Georgiou, C.D. Fluorometric determination of thiol redox state. Anal Bioanal Chem 383, 923–929 (2005). https://doi.org/10.1007/s00216-005-0095-5

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  • DOI: https://doi.org/10.1007/s00216-005-0095-5

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