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Development of a passive micromixer based on repeated fluid twisting and flattening, and its application to DNA purification

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Abstract

We have developed a three-dimensional passive micromixer based on new mixing principles, fluid twisting and flattening. This micromixer is constructed by repeating two microchannel segments, a “main channel” and a “flattened channel”, which are very different in size and are arranged perpendicularly. At the intersection of these segments the fluid inside the micromixer is twisted and then, in the flattened channel, the diffusion length is greatly reduced, achieving high mixing efficiency. Several types of micromixer were fabricated and the effect of microchannel geometry on mixing performance was evaluated. We also integrated this micromixer with a miniaturized DNA purification device, in which the concentration of the buffer solution could be rapidly changed, to perform DNA purification based on solid-phase extraction.

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Acknowledgements

This research was supported in part by Grant-in-Aids for JSPS Fellows, Scientific Research (B) (No. 16310101), and Priority Areas (A) (No. 13025216) from the Ministry of Education, Science, Sports and Culture of Japan, and by the Research Association of Micro Chemical Process Technology, Japan.

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Authors and Affiliations

  1. Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan

    Nae Yoon Lee & Masumi Yamada

  2. Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka, 599-8531, Japan

    Minoru Seki

Authors
  1. Nae Yoon Lee
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  2. Masumi Yamada
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  3. Minoru Seki
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Correspondence to Minoru Seki.

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Lee, N.Y., Yamada, M. & Seki, M. Development of a passive micromixer based on repeated fluid twisting and flattening, and its application to DNA purification. Anal Bioanal Chem 383, 776–782 (2005). https://doi.org/10.1007/s00216-005-0073-y

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  • DOI: https://doi.org/10.1007/s00216-005-0073-y

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