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Development of an on-line UV decomposition system for direct coupling of liquid chromatography to atomic-fluorescence spectrometry for selenium speciation analysis

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Abstract

Speciation analysis of four selenium species (selenite, selenate, selenocystine, and selenomethionine) has been performed by on-line coupling of liquid chromatography (LC), UV decomposition, hydride generation (HG), and atomic-fluorescence spectrometry (AFS). Because only selenite (SeIV) can generate hydrides, on-line conversion of organic and inorganic selenium species is discussed. Preliminary study showed that the use of only UV light was not sufficient to reduce selenate, because no absorption is observed for this compound at the main wavelength of the low-pressure mercury lamp (253.7 nm). Thus, new conditions based on addition of a reducing reagent (I−) were developed. Mechanisms of action are proposed to explain selenium species conversions. Because of their compatibility with on-line treatment, phosphate buffers were used for chromatographic separation on an anion exchange column (Hamilton PRP-X100). Detection limits (19–60 pg Se) and repeatability of the technique were close to those obtained by conventional quadrupole ICPMS. Applications to real samples such as water and oysters are presented and emphasize the robustness of the system.

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Acknowledgements

We thank S. Lacombe for her precious help in photochemistry experiments, and Project ECOS C01E10 and FONDECYT 1010320 (Scientific collaboration between France and Chile) and Conseil Régional d'Aquitaine for their financial support.

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Authors and Affiliations

  1. Laboratoire de Chimie Analytique Bio-Inorganique et Environnement–UMR 5034, Université de Pau et des Pays de l'Adour, 64000, Pau, France

    Stephane Simon, Aurelie Barats, Florence Pannier & Martine Potin-Gautier

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  1. Stephane Simon
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  2. Aurelie Barats
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  3. Florence Pannier
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  4. Martine Potin-Gautier
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Correspondence to Florence Pannier.

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Simon, S., Barats, A., Pannier, F. et al. Development of an on-line UV decomposition system for direct coupling of liquid chromatography to atomic-fluorescence spectrometry for selenium speciation analysis. Anal Bioanal Chem 383, 562–569 (2005). https://doi.org/10.1007/s00216-005-0056-z

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  • DOI: https://doi.org/10.1007/s00216-005-0056-z

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