Abstract
Use of classical microbiological methods to differentiate bacteria that cause gastroenteritis is cumbersome but usually very efficient. The high cost of reagents and the time required for such identifications, approximately four days, could have serious consequences, however, mainly when the patients are children, the elderly, or adults with low resistance. The search for new methods enabling rapid and reagentless differentiation of these microorganisms is, therefore, extremely relevant. In this work the main microorganisms responsible for gastroenteritis, Escherichia coli, Salmonella choleraesuis, and Shigella flexneri, were studied. For each microorganism sixty different dispersions were prepared in physiological solution. The Raman spectra of these dispersions were recorded using a diode laser operating in the near infrared region. Partial least-squares (PLS) discriminant analysis was used to differentiate among the bacteria by use of their respective Raman spectra. This approach enabled correct classification of 100% of the bacteria evaluated and unknown samples from the clinical environment, in less time (∼10 h), by use of a low-cost, portable Raman spectrometer, which can be easily used in intensive care units and clinical environments.
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Acknowledgements
The authors thank Coordenadoria de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for fellowships and financial support of their research projects. Thanks are also given to Professor C.H. Collins, for his critical reading of the manuscript.
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Mello, C., Ribeiro, D., Novaes, F. et al. Rapid differentiation among bacteria that cause gastroenteritis by use of low-resolution Raman spectroscopy and PLS discriminant analysis. Anal Bioanal Chem 383, 701–706 (2005). https://doi.org/10.1007/s00216-005-0017-6
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DOI: https://doi.org/10.1007/s00216-005-0017-6