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157-nm Laser ablation of polymeric layers for fabrication of biomolecule microarrays

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Abstract

A new methodology for protein microarray fabrication is proposed based on the ablation of polymer film using laser at 157 nm (F2). The polymer has been selected among others with the criterion of negligible protein adsorption. Improved results have been obtained by pretreatment of the polymer surface with an inert protein. The use of 157-nm laser radiation allowed very good depth control during the polymeric layer ablation process. In addition the importance of laser ablation at 157 nm is based on the fact that irradiated surfaces indicate limited chemical change due to the fact that laser ablation at 157 nm is only photochemical, thus avoiding excessive surface heating and damage. Results of protein microarray fabrication are presented to illustrate the viability of the proposed method.

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References

  1. Langer R, Tirell DA (2004) Nature 428:487–492

    Article  Google Scholar 

  2. Schena M, Shalon D, Davis RW, Brown PO (1995) Science 270:467–470

    CAS  PubMed  Google Scholar 

  3. Demers LM, Ginger DS, Park SJ, Li Z, Chung SW, Mirkin CA (2002) Science 296:1836–1838

    Article  Google Scholar 

  4. Bernard A, Renault JP, Michel B, Bosshard HR, Delamarche E (2000) Adv Mater 12:1067–1070

    Article  Google Scholar 

  5. Chiu DT, Jeon NL, Huang S, Kane RS, Wargo CJ, Choi IS, Ingber DE, Whitesides GM (2000) Proc Nat Acad Sci USA 97:2408–2413

    Article  Google Scholar 

  6. Fodor SPA, Read JL, Pirrung MC, Stryer LT, Lu A, Solas D (1991) Science 251:767–773

    Google Scholar 

  7. Cerrina F, Blattner F, Huang W, Hue Y, Green R, Singh-Gasson S, Sussman M (2002) Microelectron Eng 61–62:33–40

    Article  Google Scholar 

  8. Douvas A, Argitis P, Diakoumakos CD, Misiakos K, Dimotikali D, Kakabakos SE (2001) J Vac Sci Technol B 19:2820–2824

    Article  Google Scholar 

  9. He W, Gonsalves KE, Pickett JH, Halberstadt C (2003) Biomacromolecules 4:75–79

    Article  Google Scholar 

  10. Martelé Y, Callewaert K, Naessens K, Daele PV, Baets R, Schacht E (2003) Mater Sci Eng C 23:341–345

    Article  Google Scholar 

  11. Ostuni E, Chapman RG, Holmlin RE, Takayama S, Whitesides GM (2001) Langmuir 17:5605–5620

    Article  Google Scholar 

  12. Cefalas AC, Sarantopoulou E, Gogolides E, Argitis P (2000) Microelectron Eng 53:123–126

    Article  Google Scholar 

  13. Cefalas AC, Sarantopoulou E, Argitis P, Gogolides E (1999) Appl Phys A 69:S929-S933

    Article  Google Scholar 

  14. Lippert T, Dickinson JT (2003) Chem Rev 103:453–486

    Article  Google Scholar 

  15. Kakabakos SE, Christopoulos TK, Diamandis EP (1992) Clin Chem 38:338–342

    Google Scholar 

Download references

Acknowledgements

This work was funded from the Project G5RD-CT-2002-00744 “Microprotein”.

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Authors and Affiliations

  1. Institute of Microelectronics, NCSR “Demokritos”, Aghia Paraskevi, Athens, 15310, Greece

    Antonios M. Douvas, Konstantinos Misiakos & Panagiotis Argitis

  2. Institute of Radioisotopes and Radiodiagnostic Products, NCSR “Demokritos”, Aghia Paraskevi, Athens, 15310, Greece

    Panagiota S. Petrou & Sotirios E. Kakabakos

  3. Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, NHRF, Athens, 11635, Greece

    Evagelia Sarantopoulou, Zoe Kollia & Alkiviadis C. Cefalas

Authors
  1. Antonios M. Douvas
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  2. Panagiota S. Petrou
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  3. Sotirios E. Kakabakos
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  4. Konstantinos Misiakos
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  5. Panagiotis Argitis
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  6. Evagelia Sarantopoulou
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  7. Zoe Kollia
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  8. Alkiviadis C. Cefalas
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Correspondence to Antonios M. Douvas.

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Douvas, A.M., Petrou, P.S., Kakabakos, S.E. et al. 157-nm Laser ablation of polymeric layers for fabrication of biomolecule microarrays. Anal Bioanal Chem 381, 1027–1032 (2005). https://doi.org/10.1007/s00216-004-2985-3

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  • DOI: https://doi.org/10.1007/s00216-004-2985-3

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