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High-throughput analysis of phthalate esters in human serum by direct immersion SPME followed by isotope dilution–fast GC/MS

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Abstract

Solid-phase microextraction (SPME) coupled to gas chromatography/mass spectrometry (GC/MS) was applied to the determination of phthalate esters in human serum. The present method decreased the sample preparation time by a factor of 50 by using direct immersion SPME with an 85-μm polyacrylate fiber to extract phthalate esters from the matrix. The use of fast GC/MS further improves total analysis time when compared to other techniques. Isotope dilution was successfully applied to improve the precision, reproducibility, and repeatability of the SPME method. The linear dynamic range spans several orders of magnitude from low ppb to ppm levels, and the LOD for the method is 15 pg μL−1 on average with RSDs less than 4% for the six phthalate esters included in this study.

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Acknowledgements

The present study was conducted at the facilities of the Centers for Disease Control and Prevention (CDC) in Atlanta, GA, USA. The authors wish to thank the Oak Ridge Institute for Science and Education (ORISE) fellowship program for the post-doctoral funding of IC, Dr. James Grainger (CDC) for the synthesis of the 13C-labeled standards, and Mr. Garrick C. Clouden for his help with the experimental work.

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Authors and Affiliations

  1. Pfizer Global R&D, Groton, CT, USA

    Ivelisse Colón

  2. Department of Chemistry, Spelman College, 350 W. Spelman Lane, SW (Box 279), Atlanta, GA, 30314, USA

    Jean-Marie D. Dimandja

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  1. Ivelisse Colón
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  2. Jean-Marie D. Dimandja
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Correspondence to Jean-Marie D. Dimandja.

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Colón, I., Dimandja, JM.D. High-throughput analysis of phthalate esters in human serum by direct immersion SPME followed by isotope dilution–fast GC/MS. Anal Bioanal Chem 380, 275–283 (2004). https://doi.org/10.1007/s00216-004-2743-6

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  • DOI: https://doi.org/10.1007/s00216-004-2743-6

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