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Direct spectrometric determination of proteins in body fluids using a near-infrared cyanine dye

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Abstract

A new near-infrared (NIR) dye, 1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarbocyanine (DTCY) has been developed for the quantitation of proteins in solution. The method is based on the binding of DTCY to proteins under acidic conditions. The binding of DTCY to proteins causes a new band at 814 nm. The maximum binding number of bovine serum albumin (BSA) with DTCY was measured as 100. The linear range is 0.3–40 μg mL−1 for BSA and human serum albumin (HSA), respectively. Except for Fe2+, Cu2+, and cetyltrimethylammonium bromide, all of the examined coexisting substances show no interference in the assay. The method has been applied to the quantitation of proteins in serum and urine with recoveries between 96 and 105%.

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Acknowledgements

This work was supported by the National Science Foundation of China (No.29575206).

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Authors and Affiliations

  1. Department of Chemistry, Wuhan University, 430072, Wuhan, P.R. China

    Wei-Rong Li, Hong Wang, Ting-Xian Yang & Hua-Shan Zhang

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  1. Wei-Rong Li
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  2. Hong Wang
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  3. Ting-Xian Yang
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  4. Hua-Shan Zhang
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Correspondence to Hua-Shan Zhang.

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Li, WR., Wang, H., Yang, TX. et al. Direct spectrometric determination of proteins in body fluids using a near-infrared cyanine dye. Anal Bioanal Chem 377, 350–355 (2003). https://doi.org/10.1007/s00216-003-2100-1

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  • DOI: https://doi.org/10.1007/s00216-003-2100-1

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