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Development of microsatellite markers in Cannabis sativa for DNA typing and genetic relatedness analyses

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Abstract

Microsatellite markers were developed for Cannabis sativa L. (marijuana) to be used for DNA typing (genotype identification) and to measure the genetic relationships between the different plants. Twelve different oligonucleotide probes were used to screen an enriched microsatellite library of Cannabis sativa in which 49% of the clones contained microsatellite sequences. Characterization of microsatellite loci in Cannabis revealed that GA/CT was the most abundant class of the isolated microsatellites representing 50% overall followed by GTT/CAA, AAG/TTC, and GAT/CTA representing 16%, 15%, and 10%, respectively. Eleven polymorphic STR markers were developed, three derived from dinucleotide motifs and eight from trinucleotide motifs. A total of 52 alleles were detected averaging 4.7 alleles/locus. The expected heterozygosity of the eleven loci ranged between 0.368 and 0.710 and the common probability of identical genotypes was 1.8×10−7. The loci identified 27 unique profiles of the 41 Cannabis samples. The 11 microsatellite markers developed in this study were found to be useful for DNA typing and for assessing genetic relatedness in Cannabis.

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Acknowledgements.

This work is part of the Masters thesis of the first author at Florida International University, Miami, Florida. The authors would like to specially thank the Dubai Police Crime Laboratory in the United Arab Emirates for providing the financial support for this project and Drs Alan Meerow and Raymond Schnell at the USDA-ARS-SHRS laboratory in Miami, Florida for the use of their facilities and for helpful discussions and review of this manuscript. The authors also thank Jason Clayton at the USDA-ARS-SHRS laboratory for technical assistance and all the USDA staff at the genetic research lab in Miami, Florida. Finally, the authors thank Dr Heather Coyle of CSFSL, USA and Dr Gary Shutler of RCMP, Canada for providing the DNA samples and the AFLP information.

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  1. Department of Chemistry, International Research Forensic Institute, Florida International University, University Park, Miami, FL, 33314, USA

    H. J. Alghanim & J. R. Almirall

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  1. H. J. Alghanim
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  2. J. R. Almirall
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Correspondence to J. R. Almirall.

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Alghanim, H.J., Almirall, J.R. Development of microsatellite markers in Cannabis sativa for DNA typing and genetic relatedness analyses. Anal Bioanal Chem 376, 1225–1233 (2003). https://doi.org/10.1007/s00216-003-1984-0

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  • DOI: https://doi.org/10.1007/s00216-003-1984-0

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