Abstract.
A novel reversed phase ion-pair micro HPLC system with on-line fluorescence detection has been developed systematically and studied for the determination of cadmium in its bio-available fractions of soil samples. In this system, a micro ODS column of 1.0 mm i.d.×150 mm length and a mobile phase containing 6 mmol L-1 8-hydroxyquinoline 5-sulphonic acid (HQS), 3 mmol L-1 cetyltrimethylammonium bromide (CTMABr), 10 mmol L-1 acetic acid-acetate buffer (pH 5.1) as well as 50% acetonitrile at 50 μL min-1 flow rate were employed to determine cadmium with a 2 μL flow cell through its fluorescence at 518 nm under 338 nm excitation. Furthermore, the composition of Cd-HQS chelate formed on the column was confirmed to be [Cd(HQS)2]2- through a log–log plot method, and then combined with the ion-pair reagent by the electrostatic force under the chromatographic condition proposed. With such a method, the detection limit of cadmium was 8.48 ng mL-1 (3σ) with 1 μL sample injection, and the linear range for the determination of cadmium was 30–800 ng mL-1 (R2=0.992). This method has been successfully applied to determination of cadmium in its bio-available fractions of BCR-483 and soil samples without interference from other coexistent metal ions. The RSD (n=6) was less than 7.3%. The results were in agreement with the indicative value for BCR-483 and those for the soil samples obtained by ICP-MS with a pretreatment of bis(1,1,3,3-tetramethylbutyl) phosphinic acid extraction.
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References
William GS, David WE, Roger MT (1978) Environ Sci Technol 12:409
Quevauviller Ph (1998) Method performance studies for speciation analysis. The Royal Society of Chemistry, Cambridge, UK
Templeton DM, Ariese F, Cornelis R, Danielsson L-G, Muntau H, Van leeuwen HP, Lobinski R (2000) Pure Appl Chem 72:1453
Viksna A, Lindgren ES (1997) Anal Chim Acta 353:307
Olsson IM, Oskarsson A (2001) Analyst 126:114
Yebra MC, Enriquez MF, Cespon RM (2000) Talanta 52:631
Sun YL, Chen MS, Wang YP (1999) Spectrosc Spectral Anal 19:601
Karunasagar D, Arunachalam J (2001) Anal Chim Acta 441:291
Sato T, Kato M, Kimijima K, Okuyama M, Chiba K (2000) Spectrochim Acta Part B 55:383
Pillips DA, Soroka K, Vithanage RS, Dasgupta PK (1986) Mikrochim Acta 1:207
Ryan DE, Pitts AE, Cassidy RM (1966) Anal Chim Acta 34:491
Dasgupta PK, Soroka K, Vithanage RS (1987) J Liq Chromatogr 10:3287
Sutheimer HS, Cabaniss SE (1995) Anal Chem 67:2342
Tsunoda K, Yagasaki T, Aizawa S, Akaiwa H, Satake K (1997) Anal Sci 13:757
Soroka K, Vithanage RS, Pillips DA, Walker B, DasguptaPK (1987) Anal Chem 59:629
Feng YQ, Shibukawa M, Oguma K (1995) Chromatographia 41:532
Feng YQ, Shibukawa M, Oguma K (1996) J Chromatogr Sci 34:425
Paull B, Twohill E, Bashir W (2000) J Chromatogr A 877:123
Ure AM, Quevauviller Ph, Muntau H, Griepink B (1993) Intern J Environ Anal Chem 51:135
Sahuquillo A, López-Sánchez JF, Rubio R, Rauret G, Thomas RP, Davidson CM, Ure AM (1999) Anal Chim Acta 382:317
Rauret G, López-Sánchez JF, Sahuquillo A, Rubio R, Davidson CM, Ure AM, Quevauviller Ph (1999) J Environ Monit 1:57
Tokalioglu S, Kartal S, Elci L (2000) Anal Chim Acta 413:33
Quevauviller P (2002) Trends Anal Chem 21:774
Petruzzelli G (1989) Agric Eco Environ 27:493
Shuman LM (1991) In: Mortvedt JJ, Cox FR, Shuman LM, Welch RM (eds) Micronutrients in agriculture, 2nd edn. Soil Science Society of America, Madison, WI, p 113
Li B, Wang QQ, Huang BL, Li SP (2001) Anal Sci 17 (supplement): i1561
Wang QQ, Huang BL, Tsunoda K, Akaiwa HY (2000) Chinese J Anal Chem 28:759
Acknowledgements.
This work was partly financially supported by the National Natural Science Foundation of China (No. 20175019), the Natural Science Foundation of Fujian Province, China (No. F00011) and Doctoral Research Foundation of Education Ministry of China (No. 20020384003). The authors thank Dr Y. Feng for his valuable suggestion and discussion, and appreciate Prof. K. Tsunoda and Dr T. Umemura for offering the micro ODS column.
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Li, B., Wang, Q., Yan, H. et al. Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples. Anal Bioanal Chem 376, 923–927 (2003). https://doi.org/10.1007/s00216-003-1966-2
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DOI: https://doi.org/10.1007/s00216-003-1966-2