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Biosorption of cadmium, copper, lead and zinc by inactive biomass of Pseudomonas Putida

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Abstract

The accumulation of Cd(II), Cu(II), Pb(II) and Zn(II) at mg L−1 concentration levels by inactive freeze-dried biomass of Pseudomonas Putida has been investigated. These metals could be efficiently removed from diluted aqueous solutions. A contact time of 10 min was sufficient to reach equilibrium. The pH has a strong effect on metal biosorption and the optimal pH values were 6.0, 5.0–6.0, 6.0–6.5 and 7.0–7.5 for Cd(II), Cu(II), Pb(II) and Zn(II) respectively. Under these conditions there was 80% removal for all metals studied. The process of biosorption can be described by a Langmuir-type adsorption model. This model accounts for 98% of the data variance. The K A and q max parameters for each metal are strongly correlated (at confidence levels greater than 98%) with the metal acidity, quantified by the constant of the corresponding M(OH)+ complex, thus confirming previous assertions by other authors.

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Authors and Affiliations

  1. Departamento de Química Analítica, Universidad de Valladolid, Facultad de Ciencias, Prado de la Magdalena, 47005 , Valladolid, Spain

    Rafael Pardo, Mar Herguedas, Enrique Barrado & Marisol Vega

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  1. Rafael Pardo
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  2. Mar Herguedas
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  3. Enrique Barrado
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  4. Marisol Vega
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Correspondence to Rafael Pardo.

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Pardo, R., Herguedas, M., Barrado, E. et al. Biosorption of cadmium, copper, lead and zinc by inactive biomass of Pseudomonas Putida . Anal Bioanal Chem 376, 26–32 (2003). https://doi.org/10.1007/s00216-003-1843-z

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  • DOI: https://doi.org/10.1007/s00216-003-1843-z

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