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Determination of methylparaben, propylparaben, triamcinolone acetonide and its degradation product in a topical cream by RP-HPLC

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Abstract

A novel reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone (occurring in formulation after long-term stability tests) and two preservatives presented in the cream—methylparaben and propylparaben, using hydrocortisone as an internal standard.

The chromatographic separation was performed on a Supelco Discovery C18 column; the mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (40:60 v/v). The analysis time was less than 9 min, at a flow rate of 0.6 mL min−1 and detection at 240 nm. The method was found to be applicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolon cream 0.1%.

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Acknowledgements

The authors gratefully acknowledge the financial support of the Grant Agency of the Ministry of Education of the Czech Republic – MSM 111600001 and the Research project LN00B125.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic

    L. Matysová, R. Hájková & P. Solich

  2. Faculty of Pharmacy, The Research Centre LN00B125, Hradec Králové, Czech Republic

    P. Solich

  3. Bochemie Group, Herbacos-Bofarma Ltd., Štrossova 239, 530 02 Pardubice, Czech Republic

    J. Šícha

Authors
  1. L. Matysová
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  2. R. Hájková
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  3. J. Šícha
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  4. P. Solich
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Correspondence to P. Solich.

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Matysová, L., Hájková, R., Šícha, J. et al. Determination of methylparaben, propylparaben, triamcinolone acetonide and its degradation product in a topical cream by RP-HPLC. Anal Bioanal Chem 376, 440–443 (2003). https://doi.org/10.1007/s00216-003-1756-x

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  • DOI: https://doi.org/10.1007/s00216-003-1756-x

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